Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma

Hye Young Kim, Hyun Kyu Kang, Joon Cho, In Duk Jung, Gun Young Yoon, Min Goo Lee, Sung Jae Shin, Won Sun Park, Jong Hwan Park, Seung Wook Ryu, Yeong Min Park, Ji Chang You

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5 Citations (Scopus)

Abstract

Dendritic cells play an important role in determining whether naïve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-β production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.

Original languageEnglish
Pages (from-to)178-183
Number of pages6
JournalBMB reports
Volume48
Issue number3
DOIs
Publication statusPublished - 2015

Keywords

  • Asthma
  • Dendritic cells
  • GATA-3
  • HspX
  • Mycobacterium tuberculosis
  • T-bet

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Kim, H. Y., Kang, H. K., Cho, J., Jung, I. D., Yoon, G. Y., Lee, M. G., Shin, S. J., Park, W. S., Park, J. H., Ryu, S. W., Park, Y. M., & You, J. C. (2015). Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma. BMB reports, 48(3), 178-183. https://doi.org/10.5483/BMBRep.2015.48.3.257