Heat shock proteins and autophagy in rats with cerulein-induced acute pancreatitis

Jin Nam Kim, Hong Sik Lee, Soo Hyung Ryu, You Sun Kim, Jeong Seop Moon, Chang Duck Kim, In Youb Chang, Sang Pill Yoon

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Background/Aims: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. Methods: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 μg/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. Results: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. Conclusions: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy.

Original languageEnglish
Pages (from-to)513-520
Number of pages8
JournalGut and liver
Volume5
Issue number4
DOIs
Publication statusPublished - 2011 Dec
Externally publishedYes

Keywords

  • Acute pancreatitis
  • Autophagy
  • Heat shock protein

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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