Hemodynamic correlation imaging of the mouse brain for application in unilateral neurodegenerative diseases

Seung Ho Paik, Sedef Erdogan, Zephaniah Phillips V, Youngkyu Kim, Kang Il Song, Sunghee Estelle Park, Youngwoon Choi, Inchan Youn, Beop-Min Kim

Research output: Contribution to journalArticle

Abstract

We developed a single-camera two-channel hemodynamic imaging system that uses near-infrared light to monitor the mouse brain in vivo with an exposed, un-thinned, and intact skull to explore the effect of Parkinson’s disease on the resting state functional connectivity of the brain. To demonstrate our system’s ability to monitor cerebral hemodynamics, we first performed direct electrical stimulation of an anesthetized healthy mouse brain and detected hemodynamic changes localized to the stimulated area. Subsequently, we developed a unilaterally lesioned 6-hydroxydopamine (hemi-parkinsonian) mouse model and detected the differences in functional connectivity between the normal and hemi-parkinsonian mouse brains by comparing the hemispheric hemodynamic correlations during the resting state. Seed-based correlation for the oxy-hemoglobin channel from the left and right hemispheres of healthy mice was much higher and more symmetric than in hemi-parkinsonian mice. Through a k-means clustering of the hemodynamic signals, the healthy mouse brains were segmented according to brain region, but the hemi-parkinsonian mice did not show a similar segmentation. Overall, this study highlights the development of a spatial multiplexing hemodynamic imaging system that reveals the resting state hemodynamic connectivity in healthy and hemi-parkinsonian mice.

Original languageEnglish
Article number#349727
Pages (from-to)1736-1749
Number of pages14
JournalBiomedical Optics Express
Volume10
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

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hemodynamics
Neuroimaging
Neurodegenerative Diseases
brain
mice
Hemodynamics
Brain
Parkinson disease
oxyhemoglobin
skull
Oxidopamine
hemispheres
stimulation
multiplexing
Skull
Electric Stimulation
Cluster Analysis
Parkinson Disease
seeds
Seeds

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Hemodynamic correlation imaging of the mouse brain for application in unilateral neurodegenerative diseases. / Paik, Seung Ho; Erdogan, Sedef; Phillips V, Zephaniah; Kim, Youngkyu; Song, Kang Il; Park, Sunghee Estelle; Choi, Youngwoon; Youn, Inchan; Kim, Beop-Min.

In: Biomedical Optics Express, Vol. 10, No. 4, #349727, 01.04.2019, p. 1736-1749.

Research output: Contribution to journalArticle

Paik, Seung Ho ; Erdogan, Sedef ; Phillips V, Zephaniah ; Kim, Youngkyu ; Song, Kang Il ; Park, Sunghee Estelle ; Choi, Youngwoon ; Youn, Inchan ; Kim, Beop-Min. / Hemodynamic correlation imaging of the mouse brain for application in unilateral neurodegenerative diseases. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 4. pp. 1736-1749.
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