Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells

Mini Jeong, Jaewook Cho, Jong Il Shin, Yong Joon Jeon, Jin Hyun Kim, Sung-Joon Lee, Eun Soo Kim, Kyungho Lee

Research output: Contribution to journalArticle

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Abstract

Ethnopharmacological relevance The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. Materials and methods MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L.; cv. Cheungsam/Cannabaceae) oil by investigating cell viability, apoptosis, lipid accumulation, oxidative stress, and endoplasmic reticulum (ER) stress-induced apoptosis. Results HO treatment reduced the survival rate of MH7A cells and promoted apoptotic cell death in a time- and dose-dependent manner. Both lipid accumulation and the level of intracellular reactive oxygen species (ROS) increased in HO-treated MH7A cells. Co-treatment with the antioxidant Tiron effectively abrogated the cytotoxic effects of HO; the ROS level was reduced, cell viability was recovered, and apoptotic cell death was significantly diminished. Moreover, HO-treated cells exhibited increased expression of the major ER stress markers, glucose-regulated protein 78 and C/EBP homologous protein (CHOP). The siRNA-mediated knockdown of CHOP prevented HO-induced apoptosis. Conclusions Our results suggest that HO treatment induced lipid accumulation, ROS production, CHOP expression, and apoptosis in MH7A cells, and that CHOP functions as an anti-rheumatoid factor downstream of HO in MH7A cells.

Original languageEnglish
Pages (from-to)745-752
Number of pages8
JournalJournal of Ethnopharmacology
Volume154
Issue number3
DOIs
Publication statusPublished - 2014 Jul 3

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Transcription Factor CHOP
Reactive Oxygen Species
Rheumatoid Arthritis
Oils
Fibroblasts
Apoptosis
Endoplasmic Reticulum Stress
Cannabis
Lipids
Cannabaceae
Cell Survival
Cell Death
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Rheumatoid Factor
Traditional Medicine
Atopic Dermatitis
Therapeutics
Protein C
Unsaturated Fatty Acids
Small Interfering RNA

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells. / Jeong, Mini; Cho, Jaewook; Shin, Jong Il; Jeon, Yong Joon; Kim, Jin Hyun; Lee, Sung-Joon; Kim, Eun Soo; Lee, Kyungho.

In: Journal of Ethnopharmacology, Vol. 154, No. 3, 03.07.2014, p. 745-752.

Research output: Contribution to journalArticle

Jeong, Mini ; Cho, Jaewook ; Shin, Jong Il ; Jeon, Yong Joon ; Kim, Jin Hyun ; Lee, Sung-Joon ; Kim, Eun Soo ; Lee, Kyungho. / Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells. In: Journal of Ethnopharmacology. 2014 ; Vol. 154, No. 3. pp. 745-752.
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abstract = "Ethnopharmacological relevance The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. Materials and methods MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L.; cv. Cheungsam/Cannabaceae) oil by investigating cell viability, apoptosis, lipid accumulation, oxidative stress, and endoplasmic reticulum (ER) stress-induced apoptosis. Results HO treatment reduced the survival rate of MH7A cells and promoted apoptotic cell death in a time- and dose-dependent manner. Both lipid accumulation and the level of intracellular reactive oxygen species (ROS) increased in HO-treated MH7A cells. Co-treatment with the antioxidant Tiron effectively abrogated the cytotoxic effects of HO; the ROS level was reduced, cell viability was recovered, and apoptotic cell death was significantly diminished. Moreover, HO-treated cells exhibited increased expression of the major ER stress markers, glucose-regulated protein 78 and C/EBP homologous protein (CHOP). The siRNA-mediated knockdown of CHOP prevented HO-induced apoptosis. Conclusions Our results suggest that HO treatment induced lipid accumulation, ROS production, CHOP expression, and apoptosis in MH7A cells, and that CHOP functions as an anti-rheumatoid factor downstream of HO in MH7A cells.",
keywords = "Apoptosis, ER stress, Hempseed oil, Polyunsaturated fatty acids, Rheumatoid arthritis, Synovial cell",
author = "Mini Jeong and Jaewook Cho and Shin, {Jong Il} and Jeon, {Yong Joon} and Kim, {Jin Hyun} and Sung-Joon Lee and Kim, {Eun Soo} and Kyungho Lee",
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T1 - Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells

AU - Jeong, Mini

AU - Cho, Jaewook

AU - Shin, Jong Il

AU - Jeon, Yong Joon

AU - Kim, Jin Hyun

AU - Lee, Sung-Joon

AU - Kim, Eun Soo

AU - Lee, Kyungho

PY - 2014/7/3

Y1 - 2014/7/3

N2 - Ethnopharmacological relevance The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. Materials and methods MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L.; cv. Cheungsam/Cannabaceae) oil by investigating cell viability, apoptosis, lipid accumulation, oxidative stress, and endoplasmic reticulum (ER) stress-induced apoptosis. Results HO treatment reduced the survival rate of MH7A cells and promoted apoptotic cell death in a time- and dose-dependent manner. Both lipid accumulation and the level of intracellular reactive oxygen species (ROS) increased in HO-treated MH7A cells. Co-treatment with the antioxidant Tiron effectively abrogated the cytotoxic effects of HO; the ROS level was reduced, cell viability was recovered, and apoptotic cell death was significantly diminished. Moreover, HO-treated cells exhibited increased expression of the major ER stress markers, glucose-regulated protein 78 and C/EBP homologous protein (CHOP). The siRNA-mediated knockdown of CHOP prevented HO-induced apoptosis. Conclusions Our results suggest that HO treatment induced lipid accumulation, ROS production, CHOP expression, and apoptosis in MH7A cells, and that CHOP functions as an anti-rheumatoid factor downstream of HO in MH7A cells.

AB - Ethnopharmacological relevance The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. Materials and methods MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L.; cv. Cheungsam/Cannabaceae) oil by investigating cell viability, apoptosis, lipid accumulation, oxidative stress, and endoplasmic reticulum (ER) stress-induced apoptosis. Results HO treatment reduced the survival rate of MH7A cells and promoted apoptotic cell death in a time- and dose-dependent manner. Both lipid accumulation and the level of intracellular reactive oxygen species (ROS) increased in HO-treated MH7A cells. Co-treatment with the antioxidant Tiron effectively abrogated the cytotoxic effects of HO; the ROS level was reduced, cell viability was recovered, and apoptotic cell death was significantly diminished. Moreover, HO-treated cells exhibited increased expression of the major ER stress markers, glucose-regulated protein 78 and C/EBP homologous protein (CHOP). The siRNA-mediated knockdown of CHOP prevented HO-induced apoptosis. Conclusions Our results suggest that HO treatment induced lipid accumulation, ROS production, CHOP expression, and apoptosis in MH7A cells, and that CHOP functions as an anti-rheumatoid factor downstream of HO in MH7A cells.

KW - Apoptosis

KW - ER stress

KW - Hempseed oil

KW - Polyunsaturated fatty acids

KW - Rheumatoid arthritis

KW - Synovial cell

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JF - Journal of Ethnopharmacology

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