Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

Sung Eun Kim, Deok Won Lee, Young Pil Yun, Kyu Sik Shim, Daniel I. Jeon, Jin Kyu Rhee, Hak Jun Kim, Kyeongsoon Park

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.

    Original languageEnglish
    Article number025004
    JournalBiomedical Materials (Bristol)
    Volume11
    Issue number2
    DOIs
    Publication statusPublished - 2016 Mar 10

    Keywords

    • adipose-derived stem cells
    • heparin
    • hydroxyapatite nanoparticles
    • lactoferrin delivery
    • osteogenic differentiation

    ASJC Scopus subject areas

    • Bioengineering
    • Biomaterials
    • Biomedical Engineering

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