Heparinized micropatterned surfaces for the spatial control of human mesenchymal stem cells

Jin Woo Bae, Jong Hoon Choi, Tae Eun Kim, Ki Dong Park, Ji Youn Kim, Yongdoo Park, Kyung Sun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, a heparinized micropattern surface was prepared for the spatial control of human mesenchymal stem cells (hMSCs) that can differentiate into the desired tissues. Poly(styrene-co-vinylbenzyl N,N-diethyl- dithiocarbamate) (poly(ST-co-VBDC)) was synthesized as a photoreactive polymer; poly(ethylene glycol) methacrylate (PEGMA) was polymerized on the poly(ST-co-VBDC) coated surface by UV irradiation. XPS spectra revealed the residual DC moieties on the PEGMA-grafted surface and the linear chain growth of PEGMA was monitored according to irradiation time. After chemical immobilization of heparin onto this PEGMA surface, surface micropatterning was carried out by additional photopolymerization of PEGMA using a photomask. After incubation for 4 hour, the hMSCs adhered to the heparinized surface, while the hydrophilic PEGMA surface demonstrated no cell adhesion even after basic fibroblast growth factor (bFGF) treatment. Good alignment of hMSCs on the pattern-surface was distinctly observed along micron-sized grooves due to the presence of both heparin and bFGF. This heparinized micropattern surface can be used to study in vitro hMSCs responses with various heparin-binding growth factors in tissue engineering fields as well as cellular array for the spatial control of hMSCs.

Original languageEnglish
Pages (from-to)493-506
Number of pages14
JournalJournal of Bioactive and Compatible Polymers
Volume24
Issue number6
DOIs
Publication statusPublished - 2009 Nov 1

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Keywords

  • Basic fibroblast growth factor
  • Heparin
  • Human mesenchymal stem cells
  • PEGMA photograft
  • Surface-micropatterning cells
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry
  • Bioengineering

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