Heparin/poly(l-lysine) nanoparticle-coated polymeric microspheres for stem-cell therapy

Kun Na, Sungwon Kim, Kyeongsoon Park, Kwang Meyung Kim, Gyun Woo Dae, Ick Chan Kwon, Hyung Min Chung, Keun Hong Park

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Three-dimensional microspheres have been used extensively in several biomaterials fields, in applications such as tissue-regeneration scaffolds and drug delivery systems. To apply these biomaterials as novel cell therapeutic agents, we have devised a novel method for the fabrication of nanostructured 3D scaffolds consisting of heparin/poly(l-lysine) nanoparticles on the surface of polymeric microspheres, attached via a layer-by-layer (LbL) system. The initial step of this strategy involves the creation of heparin/poly(l-lysine) nanoparticles, which were simply produced as polyion complex micelles (PICM) with diameters of 200-500 nm. In the second step, the heparin/poly(l-lysine) nanoparticles were coated onto positively charged poly(lactic-co-glycolic acid) (PLGA) pretreated with polyethyleneimine (PEI). The production of the heparin/poly(l-lysine) nanoparticles and their subsequent coating onto PLGA microspheres represents a novel method for the functionalization of the polymeric matrix, which requires the cellular active surfaces of nanoscaled heparinized surfaces as 3D scaffolds, thus creating a better microenvironment of cell adhesion and growth for use in cell therapy applications.

Original languageEnglish
Pages (from-to)5788-5789
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number18
DOIs
Publication statusPublished - 2007 May 9
Externally publishedYes

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Cell- and Tissue-Based Therapy
Stem cells
Microspheres
Nanoparticles
Lysine
Heparin
Stem Cells
Biocompatible Materials
Biomaterials
Scaffolds
Tissue Scaffolds
Polyethyleneimine
Tissue regeneration
Acids
Cell adhesion
Cell growth
Micelles
Drug Delivery Systems
Scaffolds (biology)
Cell Adhesion

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Heparin/poly(l-lysine) nanoparticle-coated polymeric microspheres for stem-cell therapy. / Na, Kun; Kim, Sungwon; Park, Kyeongsoon; Kim, Kwang Meyung; Dae, Gyun Woo; Kwon, Ick Chan; Chung, Hyung Min; Park, Keun Hong.

In: Journal of the American Chemical Society, Vol. 129, No. 18, 09.05.2007, p. 5788-5789.

Research output: Contribution to journalArticle

Na, Kun ; Kim, Sungwon ; Park, Kyeongsoon ; Kim, Kwang Meyung ; Dae, Gyun Woo ; Kwon, Ick Chan ; Chung, Hyung Min ; Park, Keun Hong. / Heparin/poly(l-lysine) nanoparticle-coated polymeric microspheres for stem-cell therapy. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 18. pp. 5788-5789.
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