Hepatic differentiation from human embryonic stem cells using stromal cells

Young-Dong Yu, Ki Hun Kim, Sung Gyu Lee, Sang Yong Choi, Young Chul Kim, Kwan Soo Byun, In Ho Cha, Kye Yoon Park, Cheul Hyung Cho, Dong Ho Choi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.

Original languageEnglish
JournalJournal of Surgical Research
Volume170
Issue number2
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Stromal Cells
Hepatocytes
Liver
Feeder Cells
Endoderm
Mitomycin
Human Embryonic Stem Cells
Stem Cells
Embryoid Bodies
Organogenesis
Cell Transplantation
Coculture Techniques
Albumins

Keywords

  • hepatocytes
  • human embryonic stem cells
  • stromal cells

ASJC Scopus subject areas

  • Surgery

Cite this

Hepatic differentiation from human embryonic stem cells using stromal cells. / Yu, Young-Dong; Kim, Ki Hun; Lee, Sung Gyu; Choi, Sang Yong; Kim, Young Chul; Byun, Kwan Soo; Cha, In Ho; Park, Kye Yoon; Cho, Cheul Hyung; Choi, Dong Ho.

In: Journal of Surgical Research, Vol. 170, No. 2, 01.10.2011.

Research output: Contribution to journalArticle

Yu, Young-Dong ; Kim, Ki Hun ; Lee, Sung Gyu ; Choi, Sang Yong ; Kim, Young Chul ; Byun, Kwan Soo ; Cha, In Ho ; Park, Kye Yoon ; Cho, Cheul Hyung ; Choi, Dong Ho. / Hepatic differentiation from human embryonic stem cells using stromal cells. In: Journal of Surgical Research. 2011 ; Vol. 170, No. 2.
@article{5de1ea2ff2914b7189ca9e410f7850f0,
title = "Hepatic differentiation from human embryonic stem cells using stromal cells",
abstract = "Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.",
keywords = "hepatocytes, human embryonic stem cells, stromal cells",
author = "Young-Dong Yu and Kim, {Ki Hun} and Lee, {Sung Gyu} and Choi, {Sang Yong} and Kim, {Young Chul} and Byun, {Kwan Soo} and Cha, {In Ho} and Park, {Kye Yoon} and Cho, {Cheul Hyung} and Choi, {Dong Ho}",
year = "2011",
month = "10",
day = "1",
doi = "10.1016/j.jss.2011.06.032",
language = "English",
volume = "170",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Hepatic differentiation from human embryonic stem cells using stromal cells

AU - Yu, Young-Dong

AU - Kim, Ki Hun

AU - Lee, Sung Gyu

AU - Choi, Sang Yong

AU - Kim, Young Chul

AU - Byun, Kwan Soo

AU - Cha, In Ho

AU - Park, Kye Yoon

AU - Cho, Cheul Hyung

AU - Choi, Dong Ho

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.

AB - Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.

KW - hepatocytes

KW - human embryonic stem cells

KW - stromal cells

UR - http://www.scopus.com/inward/record.url?scp=80052796039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052796039&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2011.06.032

DO - 10.1016/j.jss.2011.06.032

M3 - Article

C2 - 21816427

AN - SCOPUS:80052796039

VL - 170

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -