Hepatic differentiation from human embryonic stem cells using stromal cells

Young Dong Yu, Ki Hun Kim, Sung Gyu Lee, Sang Yong Choi, Young Chul Kim, Kwan Su Byun, In Ho Cha, Kye Yoon Park, Cheul Hyung Cho, Dong Ho Choi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.

Original languageEnglish
Pages (from-to)e253-e261
JournalJournal of Surgical Research
Volume170
Issue number2
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • hepatocytes
  • human embryonic stem cells
  • stromal cells

ASJC Scopus subject areas

  • Surgery

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  • Cite this

    Yu, Y. D., Kim, K. H., Lee, S. G., Choi, S. Y., Kim, Y. C., Byun, K. S., Cha, I. H., Park, K. Y., Cho, C. H., & Choi, D. H. (2011). Hepatic differentiation from human embryonic stem cells using stromal cells. Journal of Surgical Research, 170(2), e253-e261. https://doi.org/10.1016/j.jss.2011.06.032