Hepatic immunophenotyping for streptozotocin-induced hyperglycemia in mice

Young-Sun Lee, Hyuk Soo Eun, So Yeon Kim, Jong Min Jeong, Wonhyo Seo, Jin Seok Byun, Won Il Jeong, Hyon Seung Yi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Emerging evidence revealed that diabetes induces abnormal immune responses that result in serious complications in organs. However, the effect of hyperglycemia on hepatic immunity remains obscure. We evaluated the population and function of hepatic immune cells in streptozotocin (STZ)-induced hyperglycemic mice. CC chemokine receptor 2 (CCR2)-knockout mice and mice with a depletion of regulatory T cells (DEREG) were used to investigate the migration and role of regulatory T cells (Tregs) in hyperglycemic mice. The inflammatory cytokines and hepatic transaminase levels were significantly increased in the hyperglycemic mice. The population and number of infiltrating monocytes, granulocytes, and Tregs were enhanced in the livers of the hyperglycemic mice. Hepatic monocytes other than macrophages showed the increased expression of inflammatory cytokines and chemokines in the hyperglycemic mice. The CCR2 knockout and DEREG chimeric mice exhibited increased populations of activated T cells and neutrophils compared to the WT chimeric mice, which promoted hepatic inflammation in the hyperglycemic mice. The migration of CCR2 knockout Tregs into the liver was significantly reduced compared to the WT Tregs. We demonstrated that hyperglycemia contributes to increase in infiltrating monocytes and Tregs, which are associated with hepatic immune dysfunction in mice. CCR2-mediated migration of Tregs regulates hyperglycemia-induced hepatic inflammation.

Original languageEnglish
Article number30656
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Jul 28

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Immunophenotyping
Streptozocin
Obese Mice
Hyperglycemia
CCR2 Receptors
Liver
Regulatory T-Lymphocytes
Monocytes
Cytokines
Population
Inflammation
Transaminases
Chemokines
Granulocytes
Knockout Mice
Hepatocytes
Immunity
Neutrophils
Macrophages
T-Lymphocytes

ASJC Scopus subject areas

  • General

Cite this

Lee, Y-S., Eun, H. S., Kim, S. Y., Jeong, J. M., Seo, W., Byun, J. S., ... Yi, H. S. (2016). Hepatic immunophenotyping for streptozotocin-induced hyperglycemia in mice. Scientific reports, 6, [30656]. https://doi.org/10.1038/srep30656

Hepatic immunophenotyping for streptozotocin-induced hyperglycemia in mice. / Lee, Young-Sun; Eun, Hyuk Soo; Kim, So Yeon; Jeong, Jong Min; Seo, Wonhyo; Byun, Jin Seok; Jeong, Won Il; Yi, Hyon Seung.

In: Scientific reports, Vol. 6, 30656, 28.07.2016.

Research output: Contribution to journalArticle

Lee, Y-S, Eun, HS, Kim, SY, Jeong, JM, Seo, W, Byun, JS, Jeong, WI & Yi, HS 2016, 'Hepatic immunophenotyping for streptozotocin-induced hyperglycemia in mice', Scientific reports, vol. 6, 30656. https://doi.org/10.1038/srep30656
Lee, Young-Sun ; Eun, Hyuk Soo ; Kim, So Yeon ; Jeong, Jong Min ; Seo, Wonhyo ; Byun, Jin Seok ; Jeong, Won Il ; Yi, Hyon Seung. / Hepatic immunophenotyping for streptozotocin-induced hyperglycemia in mice. In: Scientific reports. 2016 ; Vol. 6.
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