Hepatitis C virus NS2 protein activates cellular cyclic AMP-dependent pathways

Kyoung M. Kim, Shi N. Kwon, Ju I. Kang, Song Hee Lee, Sung Key Jang, Byung-Yoon Ahn, Yoon Ki Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chronic infection of the hepatitis C virus (HCV) leads to liver cirrhosis and cancer. The mechanism leading to viral persistence and hepatocellular carcinoma, however, has not been fully understood. In this study, we show that the HCV infection activates cellular cAMP-dependent pathways. Expression of a luciferase reporter gene controlled by a basic promoter with the cAMP response element (CRE) was significantly elevated in human hepatoma Huh-7 cells infected with the HCV JFH1. Analysis with viral subgenomic replicons indicated that the HCV NS2 protein is responsible for the effect. Furthermore, the level of cellular transcripts whose stability is known to be regulated by cAMP was specifically reduced in cells harboring NS2-expressing replicons. These results allude to the HCV NS2 protein having a novel function of regulating cellular gene expression and proliferation through the cAMP-dependent pathway.

Original languageEnglish
Pages (from-to)948-954
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number4
DOIs
Publication statusPublished - 2007 May 18

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Keywords

  • Cyclic AMP
  • Hepatitis C virus
  • Nonstructural protein 2
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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