Abstract
Chronic infection of the hepatitis C virus (HCV) leads to liver cirrhosis and cancer. The mechanism leading to viral persistence and hepatocellular carcinoma, however, has not been fully understood. In this study, we show that the HCV infection activates cellular cAMP-dependent pathways. Expression of a luciferase reporter gene controlled by a basic promoter with the cAMP response element (CRE) was significantly elevated in human hepatoma Huh-7 cells infected with the HCV JFH1. Analysis with viral subgenomic replicons indicated that the HCV NS2 protein is responsible for the effect. Furthermore, the level of cellular transcripts whose stability is known to be regulated by cAMP was specifically reduced in cells harboring NS2-expressing replicons. These results allude to the HCV NS2 protein having a novel function of regulating cellular gene expression and proliferation through the cAMP-dependent pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 948-954 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 356 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2007 May 18 |
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Keywords
- Cyclic AMP
- Hepatitis C virus
- Nonstructural protein 2
- Transcription
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Hepatitis C virus NS2 protein activates cellular cyclic AMP-dependent pathways. / Kim, Kyoung M.; Kwon, Shi N.; Kang, Ju I.; Lee, Song Hee; Jang, Sung Key; Ahn, Byung-Yoon; Kim, Yoon Ki.
In: Biochemical and Biophysical Research Communications, Vol. 356, No. 4, 18.05.2007, p. 948-954.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis C virus NS2 protein activates cellular cyclic AMP-dependent pathways
AU - Kim, Kyoung M.
AU - Kwon, Shi N.
AU - Kang, Ju I.
AU - Lee, Song Hee
AU - Jang, Sung Key
AU - Ahn, Byung-Yoon
AU - Kim, Yoon Ki
PY - 2007/5/18
Y1 - 2007/5/18
N2 - Chronic infection of the hepatitis C virus (HCV) leads to liver cirrhosis and cancer. The mechanism leading to viral persistence and hepatocellular carcinoma, however, has not been fully understood. In this study, we show that the HCV infection activates cellular cAMP-dependent pathways. Expression of a luciferase reporter gene controlled by a basic promoter with the cAMP response element (CRE) was significantly elevated in human hepatoma Huh-7 cells infected with the HCV JFH1. Analysis with viral subgenomic replicons indicated that the HCV NS2 protein is responsible for the effect. Furthermore, the level of cellular transcripts whose stability is known to be regulated by cAMP was specifically reduced in cells harboring NS2-expressing replicons. These results allude to the HCV NS2 protein having a novel function of regulating cellular gene expression and proliferation through the cAMP-dependent pathway.
AB - Chronic infection of the hepatitis C virus (HCV) leads to liver cirrhosis and cancer. The mechanism leading to viral persistence and hepatocellular carcinoma, however, has not been fully understood. In this study, we show that the HCV infection activates cellular cAMP-dependent pathways. Expression of a luciferase reporter gene controlled by a basic promoter with the cAMP response element (CRE) was significantly elevated in human hepatoma Huh-7 cells infected with the HCV JFH1. Analysis with viral subgenomic replicons indicated that the HCV NS2 protein is responsible for the effect. Furthermore, the level of cellular transcripts whose stability is known to be regulated by cAMP was specifically reduced in cells harboring NS2-expressing replicons. These results allude to the HCV NS2 protein having a novel function of regulating cellular gene expression and proliferation through the cAMP-dependent pathway.
KW - Cyclic AMP
KW - Hepatitis C virus
KW - Nonstructural protein 2
KW - Transcription
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UR - http://www.scopus.com/inward/citedby.url?scp=34047254849&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.03.070
DO - 10.1016/j.bbrc.2007.03.070
M3 - Article
C2 - 17395159
AN - SCOPUS:34047254849
VL - 356
SP - 948
EP - 954
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 4
ER -