Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

Ki Bae Hong, Dong Ouk Noh, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

Original languageEnglish
Pages (from-to)1069-1076
Number of pages8
JournalInternational Journal of Medicinal Mushrooms
Volume17
Issue number11
Publication statusPublished - 2015

Fingerprint

Basidiomycota
Agaricales
Hepatocytes
Water
Liver
Wounds and Injuries
Sulfonic Acids
Hydrogen Peroxide
Free Radicals

Keywords

  • Chaga mushroom
  • Hepatocyte
  • Inonotus obliquus
  • Liver injury
  • Medicinal mushrooms
  • Primary culture
  • Tert-butyl hydroperoxide

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Pharmacology

Cite this

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title = "Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes",
abstract = "We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50{\%} radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.",
keywords = "Chaga mushroom, Hepatocyte, Inonotus obliquus, Liver injury, Medicinal mushrooms, Primary culture, Tert-butyl hydroperoxide",
author = "Hong, {Ki Bae} and Noh, {Dong Ouk} and Yooheon Park and Suh, {Hyung Joo}",
year = "2015",
language = "English",
volume = "17",
pages = "1069--1076",
journal = "International Journal of Medicinal Mushrooms",
issn = "1521-9437",
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T1 - Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

AU - Hong, Ki Bae

AU - Noh, Dong Ouk

AU - Park, Yooheon

AU - Suh, Hyung Joo

PY - 2015

Y1 - 2015

N2 - We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

AB - We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

KW - Chaga mushroom

KW - Hepatocyte

KW - Inonotus obliquus

KW - Liver injury

KW - Medicinal mushrooms

KW - Primary culture

KW - Tert-butyl hydroperoxide

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