Hepatoprotective activity of water extracts from chaga medicinal mushroom, inonotus obliquus (Higher basidiomycetes) against tert-butyl hydroperoxide–induced oxidative liver injury in primary cultured rat hepatocytes

Ki Bae Hong, Dong Ouk Noh, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tertbutyl hydroperoxide (t-BHP)–induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2′-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p100 μg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP–induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.

Original languageEnglish
Pages (from-to)1069-1076
Number of pages8
JournalInternational Journal of Medicinal Mushrooms
Volume17
Issue number11
Publication statusPublished - 2015

Keywords

  • Chaga mushroom
  • Hepatocyte
  • Inonotus obliquus
  • Liver injury
  • Medicinal mushrooms
  • Primary culture
  • Tert-butyl hydroperoxide

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Pharmacology

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