Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum

Young Mi Kang, Kyung Soo Suk, Byung Ho Lee, Hak Sun Kim, Kwang Il Lee, Si Young Park, Hwan Mo Lee, Seong Hwan Moon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.

Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed.

Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E2, and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant.

Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.

Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).

Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalJournal of Spinal Disorders and Techniques
Volume27
Issue number7
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Ligamentum Flavum
Intervertebral Disc Displacement
Intervertebral Disc
Osteogenesis
Hypertrophy
Cytokines
Osteocalcin
Collagen Type XI
Coloring Agents
Spinal Stenosis
Immunosorbents
Messenger RNA
Collagen Type III
Interleukins
DNA
Dinoprostone
Reverse Transcription
Alkaline Phosphatase
Digestion
Interleukin-6

Keywords

  • Degeneration
  • Hypertrophy
  • Intervertebral disk
  • Ligamentum flavum
  • Ossification

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine
  • Clinical Neurology

Cite this

Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum. / Kang, Young Mi; Suk, Kyung Soo; Lee, Byung Ho; Kim, Hak Sun; Lee, Kwang Il; Park, Si Young; Lee, Hwan Mo; Moon, Seong Hwan.

In: Journal of Spinal Disorders and Techniques, Vol. 27, No. 7, 01.01.2014, p. 382-389.

Research output: Contribution to journalArticle

Kang, Young Mi ; Suk, Kyung Soo ; Lee, Byung Ho ; Kim, Hak Sun ; Lee, Kwang Il ; Park, Si Young ; Lee, Hwan Mo ; Moon, Seong Hwan. / Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum. In: Journal of Spinal Disorders and Techniques. 2014 ; Vol. 27, No. 7. pp. 382-389.
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abstract = "Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed.Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E2, and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant.Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.",
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T1 - Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum

AU - Kang, Young Mi

AU - Suk, Kyung Soo

AU - Lee, Byung Ho

AU - Kim, Hak Sun

AU - Lee, Kwang Il

AU - Park, Si Young

AU - Lee, Hwan Mo

AU - Moon, Seong Hwan

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed.Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E2, and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant.Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.

AB - Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed.Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E2, and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant.Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.

KW - Degeneration

KW - Hypertrophy

KW - Intervertebral disk

KW - Ligamentum flavum

KW - Ossification

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JO - Journal of Spinal Disorders and Techniques

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