Heterogeneous nuclear ribonucleoprotein A2B1 exerts a regulatory role in lipopolysaccharide-stimulated 38B9 B cell activation

Jisang Park, Chung Hyeon Choe, Ju Kim, Jing Shian Yang, Jin Hyun Kim, Hyon Seok Jang, Yong Suk Jang

Research output: Contribution to journalArticle

Abstract

Major histocompatibility complex (MHC) class II molecules, which are recognized for their primary function of presenting an antigen to the T cell receptor, are involved in various signaling pathways in B cell activation. We identified heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1 as an MHC class II molecule-associated protein involved in MHC class II-mediated signal transduction in lipopolysaccharide (LPS)-stimulated 38B9 B cells. Although the function of hnRNP A2B1 in the nucleus is primarily known, the level of hnRNP A2B1 in the cytoplasm was increased in LPS-stimulated 38B9 cells, while it was not detected in the cytoplasm of non-treated 38B9 cells. The silencing of hnRNP A2B1 expression using siRNA disturbed B cell maturation by regulation of mitogen-activated protein kinase signaling, NF-κB activation, and protein kinase B activation. These results suggest that hnRNP A2B1 is associated with MHC class II molecules and is involved in B cell activation signaling pathways in LPS-stimulated 38B9 cells.

Original languageEnglish
Pages (from-to)437-450
Number of pages14
JournalImmune Network
Volume17
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Heterogeneous-Nuclear Ribonucleoproteins
Lipopolysaccharides
Major Histocompatibility Complex
B-Lymphocytes
Cytoplasm
Proto-Oncogene Proteins c-akt
T-Cell Antigen Receptor
Mitogen-Activated Protein Kinases
Small Interfering RNA
Signal Transduction
Proteins

Keywords

  • B cell
  • Heterogeneous-nuclear ribonucleoproteins
  • MHC class II molecule
  • NF-κB
  • Signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Heterogeneous nuclear ribonucleoprotein A2B1 exerts a regulatory role in lipopolysaccharide-stimulated 38B9 B cell activation. / Park, Jisang; Choe, Chung Hyeon; Kim, Ju; Yang, Jing Shian; Kim, Jin Hyun; Jang, Hyon Seok; Jang, Yong Suk.

In: Immune Network, Vol. 17, No. 6, 01.12.2017, p. 437-450.

Research output: Contribution to journalArticle

Park, Jisang ; Choe, Chung Hyeon ; Kim, Ju ; Yang, Jing Shian ; Kim, Jin Hyun ; Jang, Hyon Seok ; Jang, Yong Suk. / Heterogeneous nuclear ribonucleoprotein A2B1 exerts a regulatory role in lipopolysaccharide-stimulated 38B9 B cell activation. In: Immune Network. 2017 ; Vol. 17, No. 6. pp. 437-450.
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