Heterogeneous nuclear RNP protein A1-arginine methylation during HCT-48 cell cycle

Seung Hee Park, Gil-Hong Park, Hyunmin Gu, Woo Ik Hwang, In Kyoung Lim, Woon Ki Paik, Sangduk Kim

Research output: Contribution to journalArticle

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Abstract

Protein methylase I (protein-arginine N-methyltransferase) was examined in HCT-48 cells, synchronized by serum deprivation and hydroxyurea treatment. The enzyme activity to methylate the added hnRNP protein A1 increased about 2-fold from G0 to S phase, and then decreased during G2/M phase. The enzymatically [methyl-3H]-labeled hnRNP protein A1 was identified by SDS-PAGE/fluorography, and the products were identified as N(G)-monomethylarginine and N(G),N(G)-dimethyl-(asymmetric)arginines by HPLC. Among endogenous proteins, the 20-kDa species in the extract was most intensely [methyl-3H]-labeled. This 20-kDa methylation was markedly inhibited by the addition of exogenous hnRNP protein A1, indicating that these two substrates compete for the same protein methylase. The possible role of this post-translational modification has been discussed.

Original languageEnglish
Pages (from-to)657-666
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume42
Issue number4
Publication statusPublished - 1997 Jul 1

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Methylation
Protein-Arginine N-Methyltransferases
Nuclear Proteins
Arginine
Cell Cycle
Cells
Photofluorography
Protein Methyltransferases
omega-N-Methylarginine
Hydroxyurea
G2 Phase
Enzyme activity
Post Translational Protein Processing
S Phase
Cell Division
Polyacrylamide Gel Electrophoresis
High Pressure Liquid Chromatography
Substrates
Enzymes
Serum

Keywords

  • Cell cycle
  • HCT-48
  • hnRNP protein A1
  • N(G),N(G)-dimethylarginine
  • N(G)-monomethylarginine
  • Protein-arginine methyltransferase
  • S-adenosylmethionine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Park, S. H., Park, G-H., Gu, H., Hwang, W. I., Lim, I. K., Paik, W. K., & Kim, S. (1997). Heterogeneous nuclear RNP protein A1-arginine methylation during HCT-48 cell cycle. Biochemistry and Molecular Biology International, 42(4), 657-666.

Heterogeneous nuclear RNP protein A1-arginine methylation during HCT-48 cell cycle. / Park, Seung Hee; Park, Gil-Hong; Gu, Hyunmin; Hwang, Woo Ik; Lim, In Kyoung; Paik, Woon Ki; Kim, Sangduk.

In: Biochemistry and Molecular Biology International, Vol. 42, No. 4, 01.07.1997, p. 657-666.

Research output: Contribution to journalArticle

Park, SH, Park, G-H, Gu, H, Hwang, WI, Lim, IK, Paik, WK & Kim, S 1997, 'Heterogeneous nuclear RNP protein A1-arginine methylation during HCT-48 cell cycle', Biochemistry and Molecular Biology International, vol. 42, no. 4, pp. 657-666.
Park, Seung Hee ; Park, Gil-Hong ; Gu, Hyunmin ; Hwang, Woo Ik ; Lim, In Kyoung ; Paik, Woon Ki ; Kim, Sangduk. / Heterogeneous nuclear RNP protein A1-arginine methylation during HCT-48 cell cycle. In: Biochemistry and Molecular Biology International. 1997 ; Vol. 42, No. 4. pp. 657-666.
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