Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield

Sandeep Kumar Tata, Jihye Jung, Yoon Ha Kim, Jun Young Choi, Ji Yul Jung, In Jung Lee, Jeong Sheop Shin, Stephen Beungtae Ryu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Geranylgeranyl pyrophosphate synthase (GGPS) is a key enzyme for a structurally diverse class of isoprenoid biosynthetic metabolites including gibberellins, carotenoids, chlorophylls and rubber. We expressed a chloroplast-targeted GGPS isolated from sunflower (Helianthus annuus) under control of the cauliflower mosaic virus 35S promoter in tobacco (Nicotiana tabacum). The resulting transgenic tobacco plants expressing heterologous GGPS showed remarkably enhanced growth (an increase in shoot and root biomass and height), early flowering, increased number of seed pods and greater seed yield compared with that of GUS-transgenic lines (control) or wild-type plants. The gibberellin levels in HaGGPS-transgenic plants were higher than those in control plants, indicating that the observed phenotype may result from increased gibberellin content. However, in HaGGPS-transformant tobacco plants, we did not observe the phenotypic defects such as reduced chlorophyll content and greater petiole and stalk length, which were previously reported for transgenic plants expressing gibberellin biosynthetic genes. Fast plant growth was also observed in HaGGPS-expressing Arabidopsis and dandelion plants. The results of this study suggest that GGPS expression in crop plants may yield desirable agronomic traits, including enhanced growth of shoots and roots, early flowering, greater numbers of seed pods and/or higher seed yield. This research has potential applications for fast production of plant biomass that provides commercially valuable biomaterials or bioenergy.

Original languageEnglish
Pages (from-to)29-39
Number of pages11
JournalPlant Biotechnology Journal
Volume14
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Chloroplasts
seed yield
Seeds
chloroplasts
gibberellins
Gibberellins
plant growth
flowering
Growth
Tobacco
Genetically Modified Plants
tobacco
Helianthus annuus
pods
transgenic plants
Helianthus
Chlorophyll
genetically modified organisms
Biomass

Keywords

  • Chloroplast
  • Fast growth
  • Geranylgeranyl pyrophosphate synthase
  • Gibberellins
  • Higher yield

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield. / Tata, Sandeep Kumar; Jung, Jihye; Kim, Yoon Ha; Choi, Jun Young; Jung, Ji Yul; Lee, In Jung; Shin, Jeong Sheop; Ryu, Stephen Beungtae.

In: Plant Biotechnology Journal, Vol. 14, No. 1, 01.01.2016, p. 29-39.

Research output: Contribution to journalArticle

Tata, Sandeep Kumar ; Jung, Jihye ; Kim, Yoon Ha ; Choi, Jun Young ; Jung, Ji Yul ; Lee, In Jung ; Shin, Jeong Sheop ; Ryu, Stephen Beungtae. / Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield. In: Plant Biotechnology Journal. 2016 ; Vol. 14, No. 1. pp. 29-39.
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