Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy, Seung Wook Kim, Seung Moon Park

Research output: Contribution to journalArticle

12 Citations (Scopus)


The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalJournal of Bioscience and Bioengineering
Issue number6
Publication statusPublished - 2011 Jun 1



  • Glycoside hydrolase
  • Phanerochaete chrysosporium
  • Pichia pastoris
  • Xylan
  • Xylanase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this