Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

Laxmi Prasad Thapa, Tae Jin Oh, Hei Chan Lee, Kwangkyoung Liou, Je Won Park, Yeo Joon Yoon, Jae Kyung Sohng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.

Original languageEnglish
Pages (from-to)1357-1364
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume76
Issue number6
DOIs
Publication statusPublished - 2007 Oct 1
Externally publishedYes

Fingerprint

Kanamycin
Streptomyces
Multigene Family
Genes
Kanamycin Resistance
Mass spectrometry
Cosmids
Electrospray Ionization Mass Spectrometry
Aminoglycosides
Regulator Genes
Tandem Mass Spectrometry
Liquid Chromatography
Open Reading Frames
Mass Spectrometry
Molecular Weight
High Pressure Liquid Chromatography
Anti-Bacterial Agents
Electrospray ionization
Liquid chromatography
High performance liquid chromatography

Keywords

  • Heterologous expression
  • Kanamycin
  • Streptomyces kanamyceticus ATCC 12853
  • Streptomyces venezuelae YJ003

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003. / Thapa, Laxmi Prasad; Oh, Tae Jin; Lee, Hei Chan; Liou, Kwangkyoung; Park, Je Won; Yoon, Yeo Joon; Sohng, Jae Kyung.

In: Applied Microbiology and Biotechnology, Vol. 76, No. 6, 01.10.2007, p. 1357-1364.

Research output: Contribution to journalArticle

Thapa, Laxmi Prasad ; Oh, Tae Jin ; Lee, Hei Chan ; Liou, Kwangkyoung ; Park, Je Won ; Yoon, Yeo Joon ; Sohng, Jae Kyung. / Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003. In: Applied Microbiology and Biotechnology. 2007 ; Vol. 76, No. 6. pp. 1357-1364.
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