Heterologous protein expression using a novel stress-responsive protein of E. coli RpoA as fusion expression partner

Keum Young Ahn, Jong A. Song, Kyung Yeon Han, Jin Seung Park, Hyuk Seong Seo, Jeewon Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

E. coli proteome response to the stressor 2-HEDS was analyzed through two-dimensional gel electrophoresis (2-DE), and we identified DNA-directed RNA polymerase α-subunit (RpoA) as stress-responsive protein. Even under stress situation where the total number of soluble proteins decreased by 9.8%, the synthesis level of RpoA was increased 1.5-fold. As a fusion expression partner as well as solubility enhancer, RpoA facilitated the folding and increased significantly the solubility of many aggregation-prone heterologous proteins (human minipro-insulin, human epidermal growth factor, human prepro-ghrelin, human interleukin-2, human activation induced cytidine deaminase, human glutamate decarboxylase, Pseudomonas putida cutinase, human ferritin light chain, human granulocyte colony-stimulating factor, and cold inflammatory syndrome1 protein Nacht domain) in E. coli cytoplasm. Due probably to intrinsic high folding efficiency and/or chaperone-like activity, RpoA was very effective in shielding interactive surfaces of heterologous proteins that are associated with non-specific protein-protein interaction leading to the formation of inclusion bodies. RpoA was also well suited for the production of biologically active fusion mutant of Pseudomonas putida cutinase that is of much biotechnological and commercial interest.

Original languageEnglish
Pages (from-to)859-866
Number of pages8
JournalEnzyme and Microbial Technology
Volume41
Issue number6-7
DOIs
Publication statusPublished - 2007 Nov 1

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Escherichia coli Proteins
DNA-Directed RNA Polymerases
Heat-Shock Proteins
Fusion reactions
Escherichia coli
Proteins
Pseudomonas putida
Solubility
Apoferritins
Ghrelin
Glutamate Decarboxylase
Inclusion Bodies
Electrophoresis, Gel, Two-Dimensional
Granulocyte Colony-Stimulating Factor
Proteome
Electrophoresis
Epidermal Growth Factor
Shielding
Interleukin-2
Membrane Proteins

Keywords

  • Escherichia coli BL21 proteome
  • RpoA
  • Solubility enhancer
  • Stress response

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Heterologous protein expression using a novel stress-responsive protein of E. coli RpoA as fusion expression partner. / Ahn, Keum Young; Song, Jong A.; Han, Kyung Yeon; Park, Jin Seung; Seo, Hyuk Seong; Lee, Jeewon.

In: Enzyme and Microbial Technology, Vol. 41, No. 6-7, 01.11.2007, p. 859-866.

Research output: Contribution to journalArticle

Ahn, Keum Young ; Song, Jong A. ; Han, Kyung Yeon ; Park, Jin Seung ; Seo, Hyuk Seong ; Lee, Jeewon. / Heterologous protein expression using a novel stress-responsive protein of E. coli RpoA as fusion expression partner. In: Enzyme and Microbial Technology. 2007 ; Vol. 41, No. 6-7. pp. 859-866.
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