High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L.

Jihye Kim, Won Je Lee, Tien Thanh Vu, Chan Young Jeong, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Key message: The ectopic expression ofAtDFRresults in increased accumulation of anthocyanins leading to enhanced salinity and drought stress tolerance inB. napusplants.Abstract: Flavonoids with antioxidant effects confer many additional benefits to plants. Evidence indicates that flavonoids, including anthocyanins, protect tissues against oxidative stress from various abiotic stressors. We determined whether increases in anthocyanins increased abiotic stress tolerance in Brassica napus, because the values of B. napus L. and its cultivation area are increasing worldwide. We overexpressed Arabidopsis dihydroflavonol-4-reductase (DFR) in B. napus. Increased DFR transcript levels for AtDFR-OX B. shoots correlated with higher anthocyanin accumulation. AtDFR-OX Brassica shoots exhibited lower reactive oxygen species (ROS) accumulation than wild-type (WT) shoots under high NaCl and mannitol concentrations. This was corroborated by 3,3-diaminobenzidine staining for ROS scavenging activity in 1,1-diphenyl-2-picryl-hydrazyl assays. Shoots of the AtDFR-OX B. napus lines grown in a high salt medium exhibited enhanced salt tolerance and higher chlorophyll content than similarly grown WT plants. Our observations suggested that the AtDFR gene can be effectively manipulated to modulate salinity and drought stress tolerance by directing to high accumulation of anthocyanins in oilseed plants.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalPlant Cell Reports
DOIs
Publication statusAccepted/In press - 2017 Apr 25

Fingerprint

stress tolerance
Brassica napus
salt stress
anthocyanins
shoots
drought tolerance
reactive oxygen species
flavonoids
oilseed crops
Brassica
mannitol
salt tolerance
abiotic stress
oxidative stress
Arabidopsis
salts
chlorophyll
antioxidants
assays
genes

Keywords

  • Anthocyanin
  • Antioxidant
  • Brassica napus
  • DFR
  • Salt stress

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L. / Kim, Jihye; Lee, Won Je; Vu, Tien Thanh; Jeong, Chan Young; Hong, Suk Whan; Lee, Hojoung.

In: Plant Cell Reports, 25.04.2017, p. 1-10.

Research output: Contribution to journalArticle

@article{b7c16953bfed43ffb3f32699f165a3a0,
title = "High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L.",
abstract = "Key message: The ectopic expression ofAtDFRresults in increased accumulation of anthocyanins leading to enhanced salinity and drought stress tolerance inB. napusplants.Abstract: Flavonoids with antioxidant effects confer many additional benefits to plants. Evidence indicates that flavonoids, including anthocyanins, protect tissues against oxidative stress from various abiotic stressors. We determined whether increases in anthocyanins increased abiotic stress tolerance in Brassica napus, because the values of B. napus L. and its cultivation area are increasing worldwide. We overexpressed Arabidopsis dihydroflavonol-4-reductase (DFR) in B. napus. Increased DFR transcript levels for AtDFR-OX B. shoots correlated with higher anthocyanin accumulation. AtDFR-OX Brassica shoots exhibited lower reactive oxygen species (ROS) accumulation than wild-type (WT) shoots under high NaCl and mannitol concentrations. This was corroborated by 3,3-diaminobenzidine staining for ROS scavenging activity in 1,1-diphenyl-2-picryl-hydrazyl assays. Shoots of the AtDFR-OX B. napus lines grown in a high salt medium exhibited enhanced salt tolerance and higher chlorophyll content than similarly grown WT plants. Our observations suggested that the AtDFR gene can be effectively manipulated to modulate salinity and drought stress tolerance by directing to high accumulation of anthocyanins in oilseed plants.",
keywords = "Anthocyanin, Antioxidant, Brassica napus, DFR, Salt stress",
author = "Jihye Kim and Lee, {Won Je} and Vu, {Tien Thanh} and Jeong, {Chan Young} and Hong, {Suk Whan} and Hojoung Lee",
year = "2017",
month = "4",
day = "25",
doi = "10.1007/s00299-017-2147-7",
language = "English",
pages = "1--10",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L.

AU - Kim, Jihye

AU - Lee, Won Je

AU - Vu, Tien Thanh

AU - Jeong, Chan Young

AU - Hong, Suk Whan

AU - Lee, Hojoung

PY - 2017/4/25

Y1 - 2017/4/25

N2 - Key message: The ectopic expression ofAtDFRresults in increased accumulation of anthocyanins leading to enhanced salinity and drought stress tolerance inB. napusplants.Abstract: Flavonoids with antioxidant effects confer many additional benefits to plants. Evidence indicates that flavonoids, including anthocyanins, protect tissues against oxidative stress from various abiotic stressors. We determined whether increases in anthocyanins increased abiotic stress tolerance in Brassica napus, because the values of B. napus L. and its cultivation area are increasing worldwide. We overexpressed Arabidopsis dihydroflavonol-4-reductase (DFR) in B. napus. Increased DFR transcript levels for AtDFR-OX B. shoots correlated with higher anthocyanin accumulation. AtDFR-OX Brassica shoots exhibited lower reactive oxygen species (ROS) accumulation than wild-type (WT) shoots under high NaCl and mannitol concentrations. This was corroborated by 3,3-diaminobenzidine staining for ROS scavenging activity in 1,1-diphenyl-2-picryl-hydrazyl assays. Shoots of the AtDFR-OX B. napus lines grown in a high salt medium exhibited enhanced salt tolerance and higher chlorophyll content than similarly grown WT plants. Our observations suggested that the AtDFR gene can be effectively manipulated to modulate salinity and drought stress tolerance by directing to high accumulation of anthocyanins in oilseed plants.

AB - Key message: The ectopic expression ofAtDFRresults in increased accumulation of anthocyanins leading to enhanced salinity and drought stress tolerance inB. napusplants.Abstract: Flavonoids with antioxidant effects confer many additional benefits to plants. Evidence indicates that flavonoids, including anthocyanins, protect tissues against oxidative stress from various abiotic stressors. We determined whether increases in anthocyanins increased abiotic stress tolerance in Brassica napus, because the values of B. napus L. and its cultivation area are increasing worldwide. We overexpressed Arabidopsis dihydroflavonol-4-reductase (DFR) in B. napus. Increased DFR transcript levels for AtDFR-OX B. shoots correlated with higher anthocyanin accumulation. AtDFR-OX Brassica shoots exhibited lower reactive oxygen species (ROS) accumulation than wild-type (WT) shoots under high NaCl and mannitol concentrations. This was corroborated by 3,3-diaminobenzidine staining for ROS scavenging activity in 1,1-diphenyl-2-picryl-hydrazyl assays. Shoots of the AtDFR-OX B. napus lines grown in a high salt medium exhibited enhanced salt tolerance and higher chlorophyll content than similarly grown WT plants. Our observations suggested that the AtDFR gene can be effectively manipulated to modulate salinity and drought stress tolerance by directing to high accumulation of anthocyanins in oilseed plants.

KW - Anthocyanin

KW - Antioxidant

KW - Brassica napus

KW - DFR

KW - Salt stress

UR - http://www.scopus.com/inward/record.url?scp=85018690011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018690011&partnerID=8YFLogxK

U2 - 10.1007/s00299-017-2147-7

DO - 10.1007/s00299-017-2147-7

M3 - Article

C2 - 28444442

AN - SCOPUS:85018690011

SP - 1

EP - 10

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

ER -