High-cell-density cultivation of oleaginous yeast Cryptococcus curvatus for biodiesel production using organic waste from the brewery industry

Byung Gon Ryu, Jungmin Kim, Kyochan Kim, Yoon E. Choi, Jong In Han, Ji Won Yang

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Waste spent yeast from brewery industry was used as a sole growth substrate to grow an oleaginous yeast Cryptococcus curvatus for the purpose of biodiesel production. Approximately 7. g/l/d of biomass productivity was obtained using only spent yeast (30. g/l) without additional nutrients and pretreatment of any kind. To make best use of available nutrients in the spent yeast, stepwise cultivation was carried out in a batch culture mode and the highest biomass and lipid content, which were 50.4. g/l and 37.7%, respectively, were obtained at 35:1 of C/N ratio. Lipid from C. curvatus was found to be a quality-sufficient source of oil as a transportation fuel in terms of cetane, iodine values, and oxidation stability, although the values of cold filter plugging point were less desirable. Economic evaluation revealed that the use of the spent yeast could significantly reduce the unit cost of yeast-based biodiesel production.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalBioresource technology
Volume135
DOIs
Publication statusPublished - 2013 May

Keywords

  • Biodiesel
  • Cryptococcus curvatus
  • Economic assessment
  • Spent yeast
  • Stepwise cultivation

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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