High cell density fermentation of Saccharomyces cerevisiae JUL3 in fed-batch culture for the production of β-Glucan

Young Hwan Kim, Seong Woo Kang, Jong Ho Lee, Hyo-Ihl Chang, Cheol-Won Yun, Hyun Dong Paik, Chang Won Kang, Seung Wook Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

β-Glucan is a cell wall component that is one of the most plentiful cell polysaccharides. Moreover, it has been found to have several beneficial effects on the immune system. In yeast, β-glucan is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast β-glucan. Response surface methodology (RSM) offers a potential means of optimizing process factors and medium components; it has been used to estimate the effects of medium components on cell mass production. In the present study, the optimal concentrations of molasses and corn steep liquor (CSL) in the medium were determined to be 6.4% (v/v) and 17% (v/v). The cell mass predicted by statistical analysis was 9.76 g/L after 20 h of cultivation. In a 2.5-L stirred tank reactor (STR), the cell mass produced in a batch culture was 36.5-39.3 g/L. The maximum cell mass in the fed-batch cultures of Saccharomyces cerevisiae JUL3 was 95.7 g/L using 50% molasses solution and a feed rate of 10 mL/h. The cell mass obtained in the fed-batch culture was 2.4-fold higher than that obtained in the batch culture.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume13
Issue number1
Publication statusPublished - 2007 Jan 1

Keywords

  • Fed-batch culture
  • High-cell-density fermentation
  • Molasses
  • Response surface methodology
  • Saccharomyces cerevisiae JUL3

ASJC Scopus subject areas

  • Engineering (miscellaneous)

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