High copy Rme1p suppresses iron-induced cell growth defect of Saccharomyces cerevisiae

Yong Sung Park, Cheol-Won Yun, Jae Yang Kong, Tae Hyoung Kim, Ha Chin Sung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the yeast Saccharomyces cerevisiae, iron can be toxic. Because of this phenomenon, its metabolism of iron is strictly regulated. We have constructed a model system in which cell growth is defected during periods of iron overload. When Aft1-1up protein was overexpressed with Gal10 promoter, a galactose inducible promoter, cell growth was defected and levels of CLN2 transcript decreased. However transcript levels of AFT1 and FET3 genes increased over time in a consistent manner throughout the course of AFT1-1up overexpression. We have screened to find genes to suppress cell growth defect by iron overload with YEp-derived high copy yeast genomic DNA library and found that high copy of Rme1p suppressed cell growth defects. Rme1p has been known as an activator protein of CLN2 gene expression. Taking these results together, we suggest that the yeast cell cycle is arrested at the G1 phase by iron overload via Cln2p.

Original languageEnglish
Pages (from-to)470-473
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume14
Issue number3
Publication statusPublished - 2004 Jun 1

Fingerprint

Cell growth
Yeast
Iron Overload
Saccharomyces cerevisiae
Iron
Defects
Yeasts
Growth
Genes
Genomic Library
Poisons
G1 Phase
Proteins
Galactose
Gene Library
Cell Cycle
Metabolism
Gene expression
Gene Expression
DNA

Keywords

  • Cell growth
  • Iron
  • Oxidative stress
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

High copy Rme1p suppresses iron-induced cell growth defect of Saccharomyces cerevisiae. / Park, Yong Sung; Yun, Cheol-Won; Kong, Jae Yang; Kim, Tae Hyoung; Sung, Ha Chin.

In: Journal of Microbiology and Biotechnology, Vol. 14, No. 3, 01.06.2004, p. 470-473.

Research output: Contribution to journalArticle

Park, Yong Sung ; Yun, Cheol-Won ; Kong, Jae Yang ; Kim, Tae Hyoung ; Sung, Ha Chin. / High copy Rme1p suppresses iron-induced cell growth defect of Saccharomyces cerevisiae. In: Journal of Microbiology and Biotechnology. 2004 ; Vol. 14, No. 3. pp. 470-473.
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