High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter

C. W. Lee, Man Bock Gu, H. N. Chang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalEnzyme and Microbial Technology
Volume11
Issue number1
DOIs
Publication statusPublished - 1989 Jan 1

Fingerprint

Fermenters
Cell growth
Cell membranes
Cell culture
Acetic Acid
Escherichia coli
Plasmids
Cell Membrane
Cell Culture Techniques
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter. / Lee, C. W.; Gu, Man Bock; Chang, H. N.

In: Enzyme and Microbial Technology, Vol. 11, No. 1, 01.01.1989, p. 49-54.

Research output: Contribution to journalArticle

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