High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter

Chang Woo Lee, Man Bock Gu, Ho Nam Chang

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

High-density cell culture study of recombinant Escherichia coli was carried out using a membrane cell recycle fermenter. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g l-1 and inhibited the cell growth. The stability of recombinant plasmid rapidly declined as the cell concentration increased during the recycle operation.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalEnzyme and Microbial Technology
Volume11
Issue number1
DOIs
Publication statusPublished - 1989 Jan

Keywords

  • Bioreactor
  • dense culture
  • recombinant Escherichia coli
  • recycle

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'High-density culture of Escherichia coli carrying recombinant plasmid in a membrane cell recycle fermenter'. Together they form a unique fingerprint.

Cite this