High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures

Sun A. Choi, You Kwan Oh, Jiye Lee, Sang Jun Sim, Min Eui Hong, Ji Yeon Park, Min Sik Kim, Seung Wook Kim, Jin Suk Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.

Original languageEnglish
Pages (from-to)120-126
Number of pages7
JournalBioresource Technology
Volume274
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Ionic Liquids
cyst
Ionic liquids
Lipids
Recovery
Water
lipid
temperature
water
Temperature
Hexanes
astaxanthine
ionic liquid
Antioxidants
Hexane
antioxidant
Anions
anion
Energy conservation
Negative ions

Keywords

  • Astaxanthin extraction
  • Cell disruption
  • Haematococcus pluvialis cyst
  • Ionic liquid
  • Lipid extraction

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures. / Choi, Sun A.; Oh, You Kwan; Lee, Jiye; Sim, Sang Jun; Hong, Min Eui; Park, Ji Yeon; Kim, Min Sik; Kim, Seung Wook; Lee, Jin Suk.

In: Bioresource Technology, Vol. 274, 01.02.2019, p. 120-126.

Research output: Contribution to journalArticle

@article{7f97751ffb554fd9969a7cb3d124eefe,
title = "High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures",
abstract = "Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7{\%} (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99{\%}) along with moderate lipid extractions (∼82{\%}) could be obtained.",
keywords = "Astaxanthin extraction, Cell disruption, Haematococcus pluvialis cyst, Ionic liquid, Lipid extraction",
author = "Choi, {Sun A.} and Oh, {You Kwan} and Jiye Lee and Sim, {Sang Jun} and Hong, {Min Eui} and Park, {Ji Yeon} and Kim, {Min Sik} and Kim, {Seung Wook} and Lee, {Jin Suk}",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.biortech.2018.11.082",
language = "English",
volume = "274",
pages = "120--126",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazolium-based ionic liquid/water mixtures

AU - Choi, Sun A.

AU - Oh, You Kwan

AU - Lee, Jiye

AU - Sim, Sang Jun

AU - Hong, Min Eui

AU - Park, Ji Yeon

AU - Kim, Min Sik

AU - Kim, Seung Wook

AU - Lee, Jin Suk

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.

AB - Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium ([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)2N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 °C, 60 min), almost complete astaxanthin recoveries (>99%) along with moderate lipid extractions (∼82%) could be obtained.

KW - Astaxanthin extraction

KW - Cell disruption

KW - Haematococcus pluvialis cyst

KW - Ionic liquid

KW - Lipid extraction

UR - http://www.scopus.com/inward/record.url?scp=85057229846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057229846&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2018.11.082

DO - 10.1016/j.biortech.2018.11.082

M3 - Article

C2 - 30502602

AN - SCOPUS:85057229846

VL - 274

SP - 120

EP - 126

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -