High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation

Hae Chul Park, Kyung Jun Lim, Jin Seo Park, Yong Hyun Lee, Tae Lin Huh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalBiotechnology Techniques
Volume9
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

Hydroxybutyrates
Alcaligenes
Electroporation
Plasmids
DNA
Kanamycin Resistance
Kanamycin
Host Specificity

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation. / Park, Hae Chul; Lim, Kyung Jun; Park, Jin Seo; Lee, Yong Hyun; Huh, Tae Lin.

In: Biotechnology Techniques, Vol. 9, No. 1, 01.01.1995, p. 31-34.

Research output: Contribution to journalArticle

Park, Hae Chul ; Lim, Kyung Jun ; Park, Jin Seo ; Lee, Yong Hyun ; Huh, Tae Lin. / High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation. In: Biotechnology Techniques. 1995 ; Vol. 9, No. 1. pp. 31-34.
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