High frequency transformation of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid by electroporation

Hae Chul Park, Kyung Jun Lim, Jin Seo Park, Yong Hyun Lee, Tae Lin Huh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalBiotechnology Techniques
Volume9
Issue number1
DOIs
Publication statusPublished - 1995 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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