High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor

Keesung Kim, Man Bock Gu, Do Hyun Kang, Jee Won Park, In Hong Song, Ho Sup Jung, Kahp Yang Suh

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We present an aptamer-based biosensor (aptasensor) for rapid and high-sensitive detection of oxytetracycline (OTC) antibiotic in PBS inside a Y-channel PDMS microfluidic device. The detection was made by real-time monitoring of the agglutination assay of ssDNA aptamer-conjugated polystyrene latex microspheres with proximity optical fibers. The agglutination assay was performed with serially diluted OTC antibiotic solutions using highly carboxylated polystyrene particles of 920 nm diameter conjugated with OTC-binding ssDNA aptamer. Proximity optical fibers were used to measure the increase in 45° forward light scattering of the aggregated particles by fixing them around the viewing cell of the device with stable angle and distance to the detector. The detection limit was around 100 ppb for the current aptasensor system with the detection time less than 3 min.

Original languageEnglish
Pages (from-to)3115-3120
Number of pages6
JournalElectrophoresis
Volume31
Issue number18
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • Agglutination assay
  • Aptamer
  • Microfluidic device
  • Oxytetracycline
  • Static light scattering

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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