High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor

Keesung Kim; Man Bock Gu; Do Hyun Kang; Jee Won Park; In Hong Song; Ho Sup Jung; Kahp Yang Suh

doi:10.1002/elps.201000217

High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor

Keesung Kim, Man Bock Gu, Do Hyun Kang, Jee Won Park, In Hong Song, Ho Sup Jung, Kahp Yang Suh

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We present an aptamer-based biosensor (aptasensor) for rapid and high-sensitive detection of oxytetracycline (OTC) antibiotic in PBS inside a Y-channel PDMS microfluidic device. The detection was made by real-time monitoring of the agglutination assay of ssDNA aptamer-conjugated polystyrene latex microspheres with proximity optical fibers. The agglutination assay was performed with serially diluted OTC antibiotic solutions using highly carboxylated polystyrene particles of 920 nm diameter conjugated with OTC-binding ssDNA aptamer. Proximity optical fibers were used to measure the increase in 45° forward light scattering of the aggregated particles by fixing them around the viewing cell of the device with stable angle and distance to the detector. The detection limit was around 100 ppb for the current aptasensor system with the detection time less than 3 min.

Original language	English
Pages (from-to)	3115-3120
Number of pages	6
Journal	Electrophoresis
Volume	31
Issue number	18
DOIs	https://doi.org/10.1002/elps.201000217
Publication status	Published - 2010 Sept

Keywords

Agglutination assay
Aptamer
Microfluidic device
Oxytetracycline
Static light scattering

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.201000217

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title = "High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor",

abstract = "We present an aptamer-based biosensor (aptasensor) for rapid and high-sensitive detection of oxytetracycline (OTC) antibiotic in PBS inside a Y-channel PDMS microfluidic device. The detection was made by real-time monitoring of the agglutination assay of ssDNA aptamer-conjugated polystyrene latex microspheres with proximity optical fibers. The agglutination assay was performed with serially diluted OTC antibiotic solutions using highly carboxylated polystyrene particles of 920 nm diameter conjugated with OTC-binding ssDNA aptamer. Proximity optical fibers were used to measure the increase in 45° forward light scattering of the aggregated particles by fixing them around the viewing cell of the device with stable angle and distance to the detector. The detection limit was around 100 ppb for the current aptasensor system with the detection time less than 3 min.",

keywords = "Agglutination assay, Aptamer, Microfluidic device, Oxytetracycline, Static light scattering",

author = "Keesung Kim and Gu, {Man Bock} and Kang, {Do Hyun} and Park, {Jee Won} and Song, {In Hong} and Jung, {Ho Sup} and Suh, {Kahp Yang}",

year = "2010",

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doi = "10.1002/elps.201000217",

language = "English",

volume = "31",

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AU - Kim, Keesung

AU - Gu, Man Bock

AU - Kang, Do Hyun

AU - Park, Jee Won

AU - Song, In Hong

AU - Jung, Ho Sup

AU - Suh, Kahp Yang

PY - 2010/9

Y1 - 2010/9

N2 - We present an aptamer-based biosensor (aptasensor) for rapid and high-sensitive detection of oxytetracycline (OTC) antibiotic in PBS inside a Y-channel PDMS microfluidic device. The detection was made by real-time monitoring of the agglutination assay of ssDNA aptamer-conjugated polystyrene latex microspheres with proximity optical fibers. The agglutination assay was performed with serially diluted OTC antibiotic solutions using highly carboxylated polystyrene particles of 920 nm diameter conjugated with OTC-binding ssDNA aptamer. Proximity optical fibers were used to measure the increase in 45° forward light scattering of the aggregated particles by fixing them around the viewing cell of the device with stable angle and distance to the detector. The detection limit was around 100 ppb for the current aptasensor system with the detection time less than 3 min.

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KW - Agglutination assay

KW - Aptamer

KW - Microfluidic device

KW - Oxytetracycline

KW - Static light scattering

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High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor

Abstract

Keywords

ASJC Scopus subject areas

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