HIGH-SPEED DROPLET SQUEEZING FOR T-CELL ENGINEERING

You Jeong Kim, Ha Sung Lee, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel droplet-based intracellular delivery platform is presented leveraging droplet microfluidics with mechanical cell permeabilization. A single cell and cargos were encapsulated in a droplet using a flow focusing device. Then, the droplet was accelerated by the sheath flow and passed through a single constriction at high speed, creating membrane discontinuities that facilitate the convective transport of biomolecules into the cell. Using this principle, the cell mechanoporation was achieved in a controlled and reproducible manner, allowing highly efficient, and dosage-controllable with substantially lower analyte amounts. The approach also enables vector-free delivery while maintaining high cell integrity without channel clogging.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages91-92
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • Droplet microfluidics
  • Droplet squeezing
  • Intracellular delivery
  • T-cell engineering

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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