TY - GEN
T1 - HIGH-SPEED DROPLET SQUEEZING FOR T-CELL ENGINEERING
AU - Kim, You Jeong
AU - Lee, Ha Sung
AU - Chung, Aram
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (No. 2021R1A2C2006224).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - A novel droplet-based intracellular delivery platform is presented leveraging droplet microfluidics with mechanical cell permeabilization. A single cell and cargos were encapsulated in a droplet using a flow focusing device. Then, the droplet was accelerated by the sheath flow and passed through a single constriction at high speed, creating membrane discontinuities that facilitate the convective transport of biomolecules into the cell. Using this principle, the cell mechanoporation was achieved in a controlled and reproducible manner, allowing highly efficient, and dosage-controllable with substantially lower analyte amounts. The approach also enables vector-free delivery while maintaining high cell integrity without channel clogging.
AB - A novel droplet-based intracellular delivery platform is presented leveraging droplet microfluidics with mechanical cell permeabilization. A single cell and cargos were encapsulated in a droplet using a flow focusing device. Then, the droplet was accelerated by the sheath flow and passed through a single constriction at high speed, creating membrane discontinuities that facilitate the convective transport of biomolecules into the cell. Using this principle, the cell mechanoporation was achieved in a controlled and reproducible manner, allowing highly efficient, and dosage-controllable with substantially lower analyte amounts. The approach also enables vector-free delivery while maintaining high cell integrity without channel clogging.
KW - Droplet microfluidics
KW - Droplet squeezing
KW - Intracellular delivery
KW - T-cell engineering
UR - http://www.scopus.com/inward/record.url?scp=85137004738&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85137004738
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 91
EP - 92
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -