High-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity

Dong Guk Cho, Haneul Yoo, Haein Lee, Yeol Kyo Choi, Minju Lee, Dong June Ahn, Seunghun Hong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.

Original languageEnglish
Article number1507
JournalSensors (Switzerland)
Volume18
Issue number5
DOIs
Publication statusPublished - 2018 May 10

Keywords

  • Binding affinity
  • Lateral flow
  • Reaction speed
  • Rotating disk
  • Selectivity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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