High-speed line-scanning confocal microscope for biological imaging

Seung Hwan Jung; Chang Keun Kim; Sung Bin Ju; Yong Jin Cho; Hyun Woo Jeong; Beop Min Kim

doi:10.1117/12.700460

High-speed line-scanning confocal microscope for biological imaging

Seung Hwan Jung, Chang Keun Kim, Sung Bin Ju, Yong Jin Cho, Hyun Woo Jeong, Beop Min Kim

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XIV
DOIs	https://doi.org/10.1117/12.700460
Publication status	Published - 2007
Externally published	Yes
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6443
ISSN (Print)	1605-7422

Other

Other	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Country/Territory	United States
City	San Jose, CA
Period	07/1/23 → 07/1/25

Keywords

Acousto-optic deflector (AOD)
High speed confocal
Line-scanning confocal microscope

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.700460

Cite this

High-speed line-scanning confocal microscope for biological imaging. / Jung, Seung Hwan; Kim, Chang Keun; Ju, Sung Bin et al.

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV. 2007. 644318 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6443).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Jung, SH, Kim, CK, Ju, SB, Cho, YJ, Jeong, HW & Kim, BM 2007, High-speed line-scanning confocal microscope for biological imaging. in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV., 644318, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, San Jose, CA, United States, 07/1/23. https://doi.org/10.1117/12.700460

@inproceedings{847d9fe7e8244a05a78010e677e26e75,

title = "High-speed line-scanning confocal microscope for biological imaging",

abstract = "We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.",

keywords = "Acousto-optic deflector (AOD), High speed confocal, Line-scanning confocal microscope",

author = "Jung, {Seung Hwan} and Kim, {Chang Keun} and Ju, {Sung Bin} and Cho, {Yong Jin} and Jeong, {Hyun Woo} and Kim, {Beop Min}",

year = "2007",

doi = "10.1117/12.700460",

language = "English",

isbn = "0819465569",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Three-Dimensional and Multidimensional Microscopy",

note = "Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV ; Conference date: 23-01-2007 Through 25-01-2007",

}

TY - GEN

T1 - High-speed line-scanning confocal microscope for biological imaging

AU - Jung, Seung Hwan

AU - Kim, Chang Keun

AU - Ju, Sung Bin

AU - Cho, Yong Jin

AU - Jeong, Hyun Woo

AU - Kim, Beop Min

PY - 2007

Y1 - 2007

N2 - We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

AB - We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

KW - Acousto-optic deflector (AOD)

KW - High speed confocal

KW - Line-scanning confocal microscope

UR - http://www.scopus.com/inward/record.url?scp=34247335856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247335856&partnerID=8YFLogxK

U2 - 10.1117/12.700460

DO - 10.1117/12.700460

M3 - Conference contribution

AN - SCOPUS:34247335856

SN - 0819465569

SN - 9780819465566

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Three-Dimensional and Multidimensional Microscopy

T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV

Y2 - 23 January 2007 through 25 January 2007

ER -

High-speed line-scanning confocal microscope for biological imaging

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this