High-speed line-scanning confocal microscope for biological imaging

Seung Hwan Jung, Chang Keun Kim, Sung Bin Ju, Yong Jin Cho, Hyun Woo Jeong, Beop Min Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XIV
DOIs
Publication statusPublished - 2007
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6443
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/25

Keywords

  • Acousto-optic deflector (AOD)
  • High speed confocal
  • Line-scanning confocal microscope

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Jung, S. H., Kim, C. K., Ju, S. B., Cho, Y. J., Jeong, H. W., & Kim, B. M. (2007). High-speed line-scanning confocal microscope for biological imaging. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV [644318] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6443). https://doi.org/10.1117/12.700460