Abstract
We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6443 |
| DOIs | |
| Publication status | Published - 2007 Apr 30 |
| Externally published | Yes |
| Event | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 25 |
Other
| Other | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 07/1/23 → 07/1/25 |
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Keywords
- Acousto-optic deflector (AOD)
- High speed confocal
- Line-scanning confocal microscope
ASJC Scopus subject areas
- Engineering(all)
Cite this
High-speed line-scanning confocal microscope for biological imaging. / Jung, Seung Hwan; Kim, Chang Keun; Ju, Sung Bin; Cho, Yong Jin; Jeong, Hyun Woo; Kim, Beop-Min.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6443 2007. 644318.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - High-speed line-scanning confocal microscope for biological imaging
AU - Jung, Seung Hwan
AU - Kim, Chang Keun
AU - Ju, Sung Bin
AU - Cho, Yong Jin
AU - Jeong, Hyun Woo
AU - Kim, Beop-Min
PY - 2007/4/30
Y1 - 2007/4/30
N2 - We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.
AB - We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.
KW - Acousto-optic deflector (AOD)
KW - High speed confocal
KW - Line-scanning confocal microscope
UR - http://www.scopus.com/inward/record.url?scp=34247335856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247335856&partnerID=8YFLogxK
U2 - 10.1117/12.700460
DO - 10.1117/12.700460
M3 - Conference contribution
AN - SCOPUS:34247335856
SN - 0819465569
SN - 9780819465566
VL - 6443
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -