@inproceedings{847d9fe7e8244a05a78010e677e26e75,
title = "High-speed line-scanning confocal microscope for biological imaging",
abstract = "We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.",
keywords = "Acousto-optic deflector (AOD), High speed confocal, Line-scanning confocal microscope",
author = "Jung, {Seung Hwan} and Kim, {Chang Keun} and Ju, {Sung Bin} and Cho, {Yong Jin} and Jeong, {Hyun Woo} and Kim, {Beop Min}",
year = "2007",
doi = "10.1117/12.700460",
language = "English",
isbn = "0819465569",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Three-Dimensional and Multidimensional Microscopy",
note = "Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV ; Conference date: 23-01-2007 Through 25-01-2007",
}