High-Speed Microfluidic Manipulation of Cells

Aram Chung, Soojung Claire Hur

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This chapter discusses the recent advancements in microfluidic cell manipulation techniques, which operate in a "high-speed" manner. A high-speed process is extremely crucial for cell manipulation because the process speed directly correlates with biological sample volumes that the technique is capable of analyzing. The chapter first introduces each cell manipulation technique, classifies further based on its working principle, and discusses the advantages and disadvantages of the technology. The chapter tabulates all techniques and summarizes the cell processing rate based on the individual technique to easily compare all different approaches. The chapter focuses on cell deformability-based sorting via channel constrictions and general deformability-based cell separation. Cells can be purified using their intrinsic stiffness, and much effort has been made on investigating RBC deformability. Along with cell separation, another key cell path manipulation method is cell alignment. The most widely explored cell focusing application is flow cytometry.

Original languageEnglish
Title of host publicationMicro- and Nanomanipulation Tools
Publisherwiley
Pages1-40
Number of pages40
ISBN (Electronic)9783527690237
ISBN (Print)9783527337842
DOIs
Publication statusPublished - 2015 Nov 25
Externally publishedYes

    Fingerprint

Keywords

  • Cell alignment
  • Cell separation
  • Constriction-based cell separation
  • Electrical cell manipulation
  • Magnetic cell manipulation
  • Mechanical cell manipulation
  • Microfluidics-based cell manipulation
  • Optical cell manipulation

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Chung, A., & Hur, S. C. (2015). High-Speed Microfluidic Manipulation of Cells. In Micro- and Nanomanipulation Tools (pp. 1-40). wiley. https://doi.org/10.1002/9783527690237.ch1