High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han, Kang Bin Im, Hwajoon Park, Dongsun Kim, Beop Min Kim

Research output: Contribution to journalConference articlepeer-review

Abstract

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

Original languageEnglish
Article number33
Pages (from-to)164-171
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5701
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII - San Jose, CA, United States
Duration: 2005 Jan 252005 Jan 27

Keywords

  • Acousto-optic deflector
  • Confocal microscope
  • Line scan CCD
  • Three-dimensional microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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