High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han, Kang Bin Im, Hwajoon Park, Dongsun Kim, Beop-Min Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512×512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 μm and <1 μm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsJ.-A. Conchello, C.J. Cogswell, T. Wilson
Pages164-171
Number of pages8
Volume5701
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII - San Jose, CA, United States
Duration: 2005 Jan 252005 Jan 27

Other

OtherThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII
CountryUnited States
CitySan Jose, CA
Period05/1/2505/1/27

Fingerprint

Optics
Microscopic examination
Microscopes
Cameras
Scanning
Lasers

Keywords

  • Acousto-optic deflector
  • Confocal microscope
  • Line scan CCD
  • Three-dimensional microscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Han, S., Im, K. B., Park, H., Kim, D., & Kim, B-M. (2005). High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera. In J-A. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5701, pp. 164-171). [33] https://doi.org/10.1117/12.591465

High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera. / Han, Sumin; Im, Kang Bin; Park, Hwajoon; Kim, Dongsun; Kim, Beop-Min.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / J.-A. Conchello; C.J. Cogswell; T. Wilson. Vol. 5701 2005. p. 164-171 33.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Han, S, Im, KB, Park, H, Kim, D & Kim, B-M 2005, High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera. in J-A Conchello, CJ Cogswell & T Wilson (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5701, 33, pp. 164-171, Three- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII, San Jose, CA, United States, 05/1/25. https://doi.org/10.1117/12.591465
Han S, Im KB, Park H, Kim D, Kim B-M. High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera. In Conchello J-A, Cogswell CJ, Wilson T, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5701. 2005. p. 164-171. 33 https://doi.org/10.1117/12.591465
Han, Sumin ; Im, Kang Bin ; Park, Hwajoon ; Kim, Dongsun ; Kim, Beop-Min. / High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / J.-A. Conchello ; C.J. Cogswell ; T. Wilson. Vol. 5701 2005. pp. 164-171
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