High-speed synthetic aperture microscopy for live cell imaging

Moonseok Kim, Youngwoon Choi, Christopher Fang-Yen, Yongjin Sung, Ramachandra R. Dasari, Michael S. Feld, Wonshik Choi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.

Original languageEnglish
Pages (from-to)148-150
Number of pages3
JournalOptics Letters
Volume36
Issue number2
DOIs
Publication statusPublished - 2011 Jan 15

Fingerprint

synthetic apertures
Microscopy
high speed
microscopy
cells
Lighting
Artifacts
Cholesterol
cholesterol
ribbons
artifacts
coverings
selectivity
illumination
diffraction
Direction compound

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

High-speed synthetic aperture microscopy for live cell imaging. / Kim, Moonseok; Choi, Youngwoon; Fang-Yen, Christopher; Sung, Yongjin; Dasari, Ramachandra R.; Feld, Michael S.; Choi, Wonshik.

In: Optics Letters, Vol. 36, No. 2, 15.01.2011, p. 148-150.

Research output: Contribution to journalArticle

Kim, M, Choi, Y, Fang-Yen, C, Sung, Y, Dasari, RR, Feld, MS & Choi, W 2011, 'High-speed synthetic aperture microscopy for live cell imaging', Optics Letters, vol. 36, no. 2, pp. 148-150. https://doi.org/10.1364/OL.36.000148
Kim M, Choi Y, Fang-Yen C, Sung Y, Dasari RR, Feld MS et al. High-speed synthetic aperture microscopy for live cell imaging. Optics Letters. 2011 Jan 15;36(2):148-150. https://doi.org/10.1364/OL.36.000148
Kim, Moonseok ; Choi, Youngwoon ; Fang-Yen, Christopher ; Sung, Yongjin ; Dasari, Ramachandra R. ; Feld, Michael S. ; Choi, Wonshik. / High-speed synthetic aperture microscopy for live cell imaging. In: Optics Letters. 2011 ; Vol. 36, No. 2. pp. 148-150.
@article{2629757090b746f28fa5824840651cec,
title = "High-speed synthetic aperture microscopy for live cell imaging",
abstract = "We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.",
author = "Moonseok Kim and Youngwoon Choi and Christopher Fang-Yen and Yongjin Sung and Dasari, {Ramachandra R.} and Feld, {Michael S.} and Wonshik Choi",
year = "2011",
month = "1",
day = "15",
doi = "10.1364/OL.36.000148",
language = "English",
volume = "36",
pages = "148--150",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "2",

}

TY - JOUR

T1 - High-speed synthetic aperture microscopy for live cell imaging

AU - Kim, Moonseok

AU - Choi, Youngwoon

AU - Fang-Yen, Christopher

AU - Sung, Yongjin

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

AU - Choi, Wonshik

PY - 2011/1/15

Y1 - 2011/1/15

N2 - We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.

AB - We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.

UR - http://www.scopus.com/inward/record.url?scp=79251489464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251489464&partnerID=8YFLogxK

U2 - 10.1364/OL.36.000148

DO - 10.1364/OL.36.000148

M3 - Article

VL - 36

SP - 148

EP - 150

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 2

ER -