High-throughput and rapid quantification of lipids by nanoflow UPLC-ESI-MS/MS: application to the hepatic lipids of rabbits with nonalcoholic fatty liver disease

Seul Kee Byeon, Jong Cheol Lee, Bong Chul Chung, Hong Seog Seo, Myeong Hee Moon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A rapid and high-throughput quantification method (approximately 300 lipids within 20 min) was established using nanoflow ultrahigh-pressure liquid chromatography-tandem mass spectrometry (nUPLC-ESI-MS/MS) with selective reaction monitoring (SRM) and applied to the quantitative profiling of the hepatic lipids of rabbits with different metabolic conditions that stimulate the development of nonalcoholic fatty liver disease (NAFLD). Among the metabolic conditions of rabbits in this study [inflammation (I), high-cholesterol diet (HC), and high-cholesterol diet combined with inflammation (HCI)], significant perturbation in hepatic lipidome (>3-fold and p < 0.01) was observed in the HC and HCI groups, while no single lipid showed a significant change in group I. In addition, this study revealed a dramatic increase (>2-fold) in relatively high-abundant monohexosylceramides (MHCs), sphingomyelins (SMs), and triacylglycerols (TGs) in both the HC and HCI groups, especially in MHCs as all 11 MHCs increased by larger than 3- to 12-fold. As the levels of the relatively high-abundant lipids in the above classes increased, the total lipidome level of each class increased significantly by approximately 2-fold to 5-fold. Other classes of lipids also generally increased, which was likely induced by the increase in mitogenic and nonapoptotic MHCs and SMs, as they promote cell proliferation. On the other hand, a slight decrease in the level of apoptotic ceramides (Cers) was observed, which agreed with the general increase in total lipid level. As distinct changes in hepatic lipidome were observed from HC groups, this suggests that HC or HCI is highly associated with NAFLD but not inflammation alone itself. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)4975-4985
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number18
DOIs
Publication statusPublished - 2016 Jul 1

Fingerprint

Nutrition
Liver
Cholesterol
Throughput
Diet
Rabbits
Lipids
Human computer interaction
Sphingomyelins
Inflammation
Ceramides
Liquid chromatography
Cell proliferation
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Triglycerides
Cell Proliferation
Non-alcoholic Fatty Liver Disease
Pressure

Keywords

  • Hepatic lipid
  • High-cholesterol diet
  • Inflammation
  • Nonalcoholic fatty liver disease
  • nUPLC-ESI-MS/MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

High-throughput and rapid quantification of lipids by nanoflow UPLC-ESI-MS/MS : application to the hepatic lipids of rabbits with nonalcoholic fatty liver disease. / Byeon, Seul Kee; Lee, Jong Cheol; Chung, Bong Chul; Seo, Hong Seog; Moon, Myeong Hee.

In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 18, 01.07.2016, p. 4975-4985.

Research output: Contribution to journalArticle

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abstract = "A rapid and high-throughput quantification method (approximately 300 lipids within 20 min) was established using nanoflow ultrahigh-pressure liquid chromatography-tandem mass spectrometry (nUPLC-ESI-MS/MS) with selective reaction monitoring (SRM) and applied to the quantitative profiling of the hepatic lipids of rabbits with different metabolic conditions that stimulate the development of nonalcoholic fatty liver disease (NAFLD). Among the metabolic conditions of rabbits in this study [inflammation (I), high-cholesterol diet (HC), and high-cholesterol diet combined with inflammation (HCI)], significant perturbation in hepatic lipidome (>3-fold and p < 0.01) was observed in the HC and HCI groups, while no single lipid showed a significant change in group I. In addition, this study revealed a dramatic increase (>2-fold) in relatively high-abundant monohexosylceramides (MHCs), sphingomyelins (SMs), and triacylglycerols (TGs) in both the HC and HCI groups, especially in MHCs as all 11 MHCs increased by larger than 3- to 12-fold. As the levels of the relatively high-abundant lipids in the above classes increased, the total lipidome level of each class increased significantly by approximately 2-fold to 5-fold. Other classes of lipids also generally increased, which was likely induced by the increase in mitogenic and nonapoptotic MHCs and SMs, as they promote cell proliferation. On the other hand, a slight decrease in the level of apoptotic ceramides (Cers) was observed, which agreed with the general increase in total lipid level. As distinct changes in hepatic lipidome were observed from HC groups, this suggests that HC or HCI is highly associated with NAFLD but not inflammation alone itself. [Figure not available: see fulltext.]",
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