High-throughput nanoscale lipid vesicle synthesis in a semicircular contraction-expansion array microchannel

Jisun Lee, Myung Gwon Lee, Cheulhee Jung, Youn Hee Park, Chaeyeon Song, Myung Chul Choi, Hyun Gyu Park, Je Kyun Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Towards potential applications in the field of nanomedicine, a new high-throughput synthesis method of lipid vesicles with tunable size as well as enhanced monodispersity is demonstrated using a semicircular contraction-expansion array (CEA) microchannel. Lipid vesicles are generated in the CEA microchannel by injecting lipids in isopropyl alcohol as a sample flow and phosphate buffered saline as a buffer flow, leading to spontaneous formation of lipid vesicles. In the CEA microchannel, Dean vortices cause three-dimensional (3D) lamination by continuously splitting and redirecting fluid streams, resulting in enhancement of fluid mixing. When considered only 3D laminating effect, it showed the best mixing efficiency in the range of flow rates of 12-15 mL/h. However, shear force effect also gives a strong influence on the formation of lipid vesicles, leading to the smallest size and uniform size distribution of lipid vesicles at a total flow rate of 18 mL/h. Consequently, from the interplay between high shear stress and 3D laminating effect, the lipid vesicles were generated with monodispersity and high throughput. The formation of lipid vesicles can be controlled with a total flow rate and a flow rate ratio between the sample and buffer fluids. The throughput of the lipid generation in the CEA microchannel was 10 times higher than previous works. In addition, the generated lipid vesicle populations were confirmed using a cryogenic transmission electron microscopy (cryo-TEM) technique.

Original languageEnglish
Pages (from-to)210-217
Number of pages8
JournalBiochip Journal
Volume7
Issue number3
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Microchannels
Lipids
Throughput
Flow rate
Laminating
Fluids
Buffers
Nanomedicine
Medical nanotechnology
2-Propanol
Transmission Electron Microscopy
Cryogenics
Shear stress
Phosphates
Vortex flow
Alcohols
Transmission electron microscopy

Keywords

  • Contraction-expansion array microchannel
  • High-throughput synthesis
  • Lipid vesicles
  • Microfluidics
  • Monodispersity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

High-throughput nanoscale lipid vesicle synthesis in a semicircular contraction-expansion array microchannel. / Lee, Jisun; Lee, Myung Gwon; Jung, Cheulhee; Park, Youn Hee; Song, Chaeyeon; Choi, Myung Chul; Park, Hyun Gyu; Park, Je Kyun.

In: Biochip Journal, Vol. 7, No. 3, 01.01.2013, p. 210-217.

Research output: Contribution to journalArticle

Lee, Jisun ; Lee, Myung Gwon ; Jung, Cheulhee ; Park, Youn Hee ; Song, Chaeyeon ; Choi, Myung Chul ; Park, Hyun Gyu ; Park, Je Kyun. / High-throughput nanoscale lipid vesicle synthesis in a semicircular contraction-expansion array microchannel. In: Biochip Journal. 2013 ; Vol. 7, No. 3. pp. 210-217.
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