TY - JOUR
T1 - Highly amplified detection of visceral adipose tissue-derived serpin (vaspin) using a cognate aptamer duo
AU - Ahmad Raston, Nurul Hanun
AU - Gu, Man Bock
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MEST) (Nos. 2012R1A2A2A01011056 and 2013R1A1A2021531). In addition, the authors are grateful for the financial support from the School of Life Sciences and Biotechnology for BK21 PLUS in Korea University, for open access publication of this work.
Publisher Copyright:
© 2015 The Authors.
PY - 2015/8/5
Y1 - 2015/8/5
N2 - A cognate aptamer duo for visceral adipose tissue-derived serpin (vaspin) which distinctively bind to two different sites on vaspin with high affinity and specificity were successfully developed by using graphene oxide-based systematic evolution of ligands by exponential enrichment (GO-SELEX), which offers immobilization-free screening of aptamers. The specific and simultaneous bindings of this aptamer duo (V1 and V49 aptamers) to the different sites of vaspin were confirmed by circular dichroism (CD) analysis and both sandwich-type surface plasmon resonance (SPR) and quantum dot labelled fluorescence imaging analysis (V1 aptamer serves as primary capturing aptamer and V49 aptamer as secondary signalling aptamer or vice versa). With this vaspin cognate aptamer duo on SPR platform, the detection of the target vaspin were improved to the limit of detection down to 3.5. ng/ml in buffer and 4.7. ng/ml in human serum samples. This cognate aptamer duo based biosensor could be utilized in the early diagnosis of type-2 diabetes.
AB - A cognate aptamer duo for visceral adipose tissue-derived serpin (vaspin) which distinctively bind to two different sites on vaspin with high affinity and specificity were successfully developed by using graphene oxide-based systematic evolution of ligands by exponential enrichment (GO-SELEX), which offers immobilization-free screening of aptamers. The specific and simultaneous bindings of this aptamer duo (V1 and V49 aptamers) to the different sites of vaspin were confirmed by circular dichroism (CD) analysis and both sandwich-type surface plasmon resonance (SPR) and quantum dot labelled fluorescence imaging analysis (V1 aptamer serves as primary capturing aptamer and V49 aptamer as secondary signalling aptamer or vice versa). With this vaspin cognate aptamer duo on SPR platform, the detection of the target vaspin were improved to the limit of detection down to 3.5. ng/ml in buffer and 4.7. ng/ml in human serum samples. This cognate aptamer duo based biosensor could be utilized in the early diagnosis of type-2 diabetes.
UR - http://www.scopus.com/inward/record.url?scp=84925727238&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2015.03.042
DO - 10.1016/j.bios.2015.03.042
M3 - Article
C2 - 25829224
AN - SCOPUS:84925727238
SN - 0956-5663
VL - 70
SP - 261
EP - 267
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
