Highly efficient electro-permeabilization of mammalian cells using micro-electroporation chip

Young Shik Shin, Jung Kyung Kim, Sun Hee Lim, Young Kyung Lee, Keunchang Cho, Yongku Lee, Seok Chung, Chanil Chung, Dong Chul Han, Jun Keun Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. As a more convenient and effective tool for the investigation on the mechanisms of electroporation and the enhancement of the functional efficiency, we developed a micro-electroporation chip with polydimethylsiloxane (PDMS). Owing to the transparency of PDMS, we could observe the process of Propidium Iodide (PI) uptake in real-time, which shows promise in visualization of gene activity in living cells. Furthermore, the design of the electroporation chip as a microchannel offers advantages in terms of efficiency in permeabilization of PI into SK-OV-3 cells. We also noticed the geometric effect on the degree of electroporation in microchannels with diverse channel width, which shows that the geometry can be another parameter to be considered for the electroporation when it is performed in microchannels.

Original languageEnglish
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages70-73
Number of pages4
Publication statusPublished - 2004
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: 2004 Mar 72004 Mar 11

Publication series

Name2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
Volume1

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period04/3/704/3/11

Keywords

  • Bio-MEMS
  • Electroporation
  • Microchannel
  • PDMS
  • Propidium iodide

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Shin, Y. S., Kim, J. K., Lim, S. H., Lee, Y. K., Cho, K., Lee, Y., Chung, S., Chung, C., Han, D. C., & Chang, J. K. (2004). Highly efficient electro-permeabilization of mammalian cells using micro-electroporation chip. In M. Laudon, & B. Romanowicz (Eds.), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 (pp. 70-73). (2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004; Vol. 1).