Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim, Seuk Min Ryu, Sang Tae Kim, Gayoung Baek, Daesik Kim, Kayeong Lim, Eugene Chung, Sunghyun Kim, Jin Soo Kim

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

Original languageEnglish
Pages (from-to)435-437
Number of pages3
JournalNature Biotechnology
Volume35
Issue number5
DOIs
Publication statusPublished - 2017 May 1
Externally publishedYes

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Cytidine Deaminase
Cytidine
Ribonucleoproteins
Uridine
RNA
Substitution reactions
Embryonic Structures
Genes
Messenger RNA
Electroporation
Nonsense Codon
Zygote
Microinjections
Eukaryotic Cells
Point Mutation
Base Pairing

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Kim, K., Ryu, S. M., Kim, S. T., Baek, G., Kim, D., Lim, K., ... Kim, J. S. (2017). Highly efficient RNA-guided base editing in mouse embryos. Nature Biotechnology, 35(5), 435-437. https://doi.org/10.1038/nbt.3816

Highly efficient RNA-guided base editing in mouse embryos. / Kim, Kyoungmi; Ryu, Seuk Min; Kim, Sang Tae; Baek, Gayoung; Kim, Daesik; Lim, Kayeong; Chung, Eugene; Kim, Sunghyun; Kim, Jin Soo.

In: Nature Biotechnology, Vol. 35, No. 5, 01.05.2017, p. 435-437.

Research output: Contribution to journalArticle

Kim, K, Ryu, SM, Kim, ST, Baek, G, Kim, D, Lim, K, Chung, E, Kim, S & Kim, JS 2017, 'Highly efficient RNA-guided base editing in mouse embryos', Nature Biotechnology, vol. 35, no. 5, pp. 435-437. https://doi.org/10.1038/nbt.3816
Kim K, Ryu SM, Kim ST, Baek G, Kim D, Lim K et al. Highly efficient RNA-guided base editing in mouse embryos. Nature Biotechnology. 2017 May 1;35(5):435-437. https://doi.org/10.1038/nbt.3816
Kim, Kyoungmi ; Ryu, Seuk Min ; Kim, Sang Tae ; Baek, Gayoung ; Kim, Daesik ; Lim, Kayeong ; Chung, Eugene ; Kim, Sunghyun ; Kim, Jin Soo. / Highly efficient RNA-guided base editing in mouse embryos. In: Nature Biotechnology. 2017 ; Vol. 35, No. 5. pp. 435-437.
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