Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim, Seuk Min Ryu, Sang Tae Kim, Gayoung Baek, Daesik Kim, Kayeong Lim, Eugene Chung, Sunghyun Kim, Jin Soo Kim

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

Original languageEnglish
Pages (from-to)435-437
Number of pages3
JournalNature Biotechnology
Volume35
Issue number5
DOIs
Publication statusPublished - 2017 May 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Kim, K., Ryu, S. M., Kim, S. T., Baek, G., Kim, D., Lim, K., Chung, E., Kim, S., & Kim, J. S. (2017). Highly efficient RNA-guided base editing in mouse embryos. Nature Biotechnology, 35(5), 435-437. https://doi.org/10.1038/nbt.3816