Highly magnetized encoded hydrogel microparticles with enhanced rinsing capabilities for efficient microrna detection

Wookyoung Jang, Jiwoo Kim, Seok Joon Mun, Sun Min Kim, Ki Wan Bong

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Encoded hydrogel microparticles mounting DNA probes are powerful tools for high-per-formance microRNA (miRNA) detection in terms of sensitivity, specificity, and multiplex detection capability. However, several particle rinsing steps in the assay procedure present challenges for rapid and efficient detection. To overcome this limitation, we encapsulated dense magnetic nano-particles to reduce the rinsing steps and duration via magnetic separation. A large number of magnetic nanoparticles were encapsulated into hydrogel microparticles based on a discontinuous dewetting technique combined with degassed micromolding lithography. In addition, we attached DNA probes targeting three types of miRNAs related to preeclampsia to magnetically encoded hy-drogel microparticles by post-synthesis conjugation and achieved sensitivity comparable to that of conventional nonmagnetic encoded hydrogel microparticles. To demonstrate the multiplex capability of magnetically encoded hydrogel microparticles while maintaining the advantages of the simplified rinsing process when addressing multiple samples, we conducted a triplex detection of preeclampsia-related miRNAs. In conclusion, the introduction of magnetically encoded hydrogel microparticles not only allowed efficient miRNA detection but also provided comparable sensitivity and multiplexed detectability to conventional nonmagnetic encoded hydrogel microparticles.

Original languageEnglish
Article number848
JournalBiomedicines
Volume9
Issue number7
DOIs
Publication statusPublished - 2021 Jul

Keywords

  • Magnetic hydrogel
  • MicroRNA detection
  • Microparticles
  • Preeclampsia

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

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