Highly selective and sensitive detection of neurotransmitters using receptor-modified single-walled carbon nanotube sensors

Byeongju Kim, Hyun Seok Song, Hye Jun Jin, Eun Jin Park, Sang Hun Lee, Byung Yang Lee, Tai Hyun Park, Seunghun Hong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We present receptor-modified carbon nanotube sensors for the highly selective and sensitive detection of acetylcholine (ACh), one kind of neurotransmitter. Here, we successfully expressed the M1 muscarinic acetylcholine receptor (M1 mAChR), a family of G protein-coupled receptors (GPCRs), in E. coli and coated single-walled carbon nanotube (swCNT)-field effect transistors (FETs) with lipid membrane including the receptor, enabling highly selective and sensitive ACh detection. Using this sensor, we could detect ACh at 100 pM concentration. Moreover, we showed that this sensor could selectively detect ACh among other neurotransmitters. This is the first demonstration of the real-time detection of ACh using specific binding between ACh and M1 mAChR, and it may lead to breakthroughs for various applications such as disease diagnosis and drug screening.

Original languageEnglish
Article number285501
JournalNanotechnology
Volume24
Issue number28
DOIs
Publication statusPublished - 2013 Jul 19

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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