TY - JOUR
T1 - Highly sensitive immunoassay of lung cancer marker carcinoembryonic antigen using surface-enhanced raman scattering of hollow gold nanospheres
AU - Chon, Hyangah
AU - Lee, Sangyeop
AU - Son, Sang Wook
AU - Oh, Chil Hwan
AU - Choo, Jaebum
PY - 2009/4/15
Y1 - 2009/4/15
N2 - A quick and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay technique, using hollow gold nanospheres (HGNs) and magnetic beads, has been developed. Here, HGNs show strong enhancement effects from individual particles because hot spots can be localized on the pinholes in the hollow particle structure. Thus, HGNs can be used for highly reproducible immu-noanalysis of cancer markers. Magnetic beads were used as supporting substrates for the formation of the immu-nocomplex. This SERS-based immunoassay technique overcomes the problem of slow immunoreaction caused by the diffusion-limited kinetics on a solid substrate because all of the reactions occur in solution. For the validation of our SERS immunoassay, a well-known lung cancer marker, carcinoembryonic antigen (CEA), was used as a target marker. According to our experimental results, the limit of detection (LOD) was determined to be 1-10 pg/mL, this value being about 100-1000 times more sensitive than the LOD of enzyme-linked immun-osorbent assay. Furthermore, the assay time took less than 1 h, including washing and optical detection steps.
AB - A quick and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay technique, using hollow gold nanospheres (HGNs) and magnetic beads, has been developed. Here, HGNs show strong enhancement effects from individual particles because hot spots can be localized on the pinholes in the hollow particle structure. Thus, HGNs can be used for highly reproducible immu-noanalysis of cancer markers. Magnetic beads were used as supporting substrates for the formation of the immu-nocomplex. This SERS-based immunoassay technique overcomes the problem of slow immunoreaction caused by the diffusion-limited kinetics on a solid substrate because all of the reactions occur in solution. For the validation of our SERS immunoassay, a well-known lung cancer marker, carcinoembryonic antigen (CEA), was used as a target marker. According to our experimental results, the limit of detection (LOD) was determined to be 1-10 pg/mL, this value being about 100-1000 times more sensitive than the LOD of enzyme-linked immun-osorbent assay. Furthermore, the assay time took less than 1 h, including washing and optical detection steps.
UR - http://www.scopus.com/inward/record.url?scp=65249154258&partnerID=8YFLogxK
U2 - 10.1021/ac802722c
DO - 10.1021/ac802722c
M3 - Article
C2 - 19301845
AN - SCOPUS:65249154258
VL - 81
SP - 3029
EP - 3034
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 8
ER -
