Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

Van Thuan Nguyen, Ho Bin Seo, Byoung Chan Kim, Sang Kyung Kim, Chang Seon Song, Man Bock Gu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalBiosensors and Bioelectronics
Volume86
DOIs
Publication statusPublished - 2016 Dec 15

Fingerprint

H5N1 Subtype Influenza A Virus
Biosensing Techniques
Viruses
Influenza in Birds
Orthomyxoviridae
Biosensors
Feces
Gold
Nanoparticles
Research
Amplification
Assays

Keywords

  • AuNP
  • Circular Dichroism
  • Dual aptamer
  • SPR
  • Whole virus H5Nx

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers. / Nguyen, Van Thuan; Seo, Ho Bin; Kim, Byoung Chan; Kim, Sang Kyung; Song, Chang Seon; Gu, Man Bock.

In: Biosensors and Bioelectronics, Vol. 86, 15.12.2016, p. 293-300.

Research output: Contribution to journalArticle

Nguyen, Van Thuan ; Seo, Ho Bin ; Kim, Byoung Chan ; Kim, Sang Kyung ; Song, Chang Seon ; Gu, Man Bock. / Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers. In: Biosensors and Bioelectronics. 2016 ; Vol. 86. pp. 293-300.
@article{075d3263d62e408a8b27f3ea4e013913,
title = "Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers",
abstract = "In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.",
keywords = "AuNP, Circular Dichroism, Dual aptamer, SPR, Whole virus H5Nx",
author = "Nguyen, {Van Thuan} and Seo, {Ho Bin} and Kim, {Byoung Chan} and Kim, {Sang Kyung} and Song, {Chang Seon} and Gu, {Man Bock}",
year = "2016",
month = "12",
day = "15",
doi = "10.1016/j.bios.2016.06.064",
language = "English",
volume = "86",
pages = "293--300",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers

AU - Nguyen, Van Thuan

AU - Seo, Ho Bin

AU - Kim, Byoung Chan

AU - Kim, Sang Kyung

AU - Song, Chang Seon

AU - Gu, Man Bock

PY - 2016/12/15

Y1 - 2016/12/15

N2 - In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

AB - In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8×104 to 1×104 EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of AI whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles.

KW - AuNP

KW - Circular Dichroism

KW - Dual aptamer

KW - SPR

KW - Whole virus H5Nx

UR - http://www.scopus.com/inward/record.url?scp=84978967803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978967803&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2016.06.064

DO - 10.1016/j.bios.2016.06.064

M3 - Article

VL - 86

SP - 293

EP - 300

JO - Biosensors

JF - Biosensors

SN - 0956-5663

ER -