Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: Confocal surface-enhanced Raman spectroscopic study

Taehan Park, Sangyeop Lee, Gi Hun Seong, Jaebum Choo, Eun Kyu Lee, Yang S. Kim, Won Ho Ji, Seung Yong Hwang, Dae Gab Gweon, Sang Hoon Lee

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Abstract

Rapid and highly sensitive detection of duplex dye-labelled DNA sequences in a PDMS microfluidic channel was investigated using confocal surface enhanced Raman spectroscopy (SERS). This method does not need either an immobilization procedure or a PCR amplification procedure, which are essential for a DNA microarray chip. Furthermore, Raman peaks of each dye-labelled DNA can be easily resolved since they are much narrower than the corresponding broad fluorescence bands. To find the potential applicability of confocal SERS for sensitive bio-detection in a microfluidic channel, the mixture of two different dye-labelled (TAMRA and Cy3) sex determining Y genes, SRY and SPGY1, was adsorbed on silver colloids in the alligator teeth-shaped PDMS microfluidic channel and its SERS signals were measured under flowing conditions. Its major SERS peaks were observable down to the concentration of 10-11 M. In the present study, we explore the feasibility of confocal SERS for the highly sensitive detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip.

Original languageEnglish
Pages (from-to)437-442
Number of pages6
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume5
Issue number4
DOIs
Publication statusPublished - 2005 Apr 1
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Biochemistry

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