Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins

Hye Jin Kim, Woongsun Choi, Jinsik Kim, Jungkyu Choi, Nakwon Choi, Kyo Seon Hwang

Research output: Contribution to journalArticle

Abstract

This study focused on fabricating a target-specific electrical biosensor with high sensitivity and dynamic range by embedding a hydrogel, that incorporated a much larger number of antibodies in the reaction region of the sensor, allowing all biomolecules except the target molecules to be filtered off. The biosensor is comprised of a three-dimensional interdigitated microelectrode (3D IMEs) with much higher aspect ratios than conventional IMEs and a hydrogel with a 3D mesh structure. The hydrogel increases the number of antibodies in the reaction region of the 3D IMEs by approximately 1,000-fold and enhances the impedance change induced by the specific binding of biomolecules. The hydrogel density is altered according to the target biomolecules. The impedance change by Aβ42 binding was maximized to approximately 12.135 ± 0.903% in the 3D IMEs with a ‘dense’ hydrogel. The change by prostate-specific antigen (PSA) binding was maximized in the 3D IMEs with a ‘loose’ hydrogel. Thus, the dynamic range and sensitivity for detecting Aβ42 were enhanced by a maximum of two orders and by approximately 2.58-fold compared to planar IMEs. These results demonstrate the high performance of the hydrogel-embedded 3D IMEs and its applicability for biological fluid-based diagnosis.

Original languageEnglish
Article number127190
JournalSensors and Actuators, B: Chemical
Volume302
DOIs
Publication statusPublished - 2020 Jan 1

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Hydrogel
Microelectrodes
bioinstrumentation
Biosensors
Hydrogels
Porosity
proteins
Proteins
antibodies
porosity
dynamic range
impedance
Biomolecules
sensitivity
antigens
high aspect ratio
embedding
mesh
Antibodies
fluids

Keywords

  • Amyloid beta
  • High sensitivity and selectivity
  • Porosity tunable hydrogel
  • Three-dimensional interdigitated microelectrodes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins. / Kim, Hye Jin; Choi, Woongsun; Kim, Jinsik; Choi, Jungkyu; Choi, Nakwon; Hwang, Kyo Seon.

In: Sensors and Actuators, B: Chemical, Vol. 302, 127190, 01.01.2020.

Research output: Contribution to journalArticle

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