TY - JOUR
T1 - Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins
AU - Kim, Hye Jin
AU - Choi, Woongsun
AU - Kim, Jinsik
AU - Choi, Jungkyu
AU - Choi, Nakwon
AU - Hwang, Kyo Seon
N1 - Funding Information:
This work was supported by the Korea Health Industry Development Institute (KHIDI; HI14C3319 ), the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2018R1A6A3A11041491 ).
PY - 2020/1/1
Y1 - 2020/1/1
N2 - This study focused on fabricating a target-specific electrical biosensor with high sensitivity and dynamic range by embedding a hydrogel, that incorporated a much larger number of antibodies in the reaction region of the sensor, allowing all biomolecules except the target molecules to be filtered off. The biosensor is comprised of a three-dimensional interdigitated microelectrode (3D IMEs) with much higher aspect ratios than conventional IMEs and a hydrogel with a 3D mesh structure. The hydrogel increases the number of antibodies in the reaction region of the 3D IMEs by approximately 1,000-fold and enhances the impedance change induced by the specific binding of biomolecules. The hydrogel density is altered according to the target biomolecules. The impedance change by Aβ42 binding was maximized to approximately 12.135 ± 0.903% in the 3D IMEs with a ‘dense’ hydrogel. The change by prostate-specific antigen (PSA) binding was maximized in the 3D IMEs with a ‘loose’ hydrogel. Thus, the dynamic range and sensitivity for detecting Aβ42 were enhanced by a maximum of two orders and by approximately 2.58-fold compared to planar IMEs. These results demonstrate the high performance of the hydrogel-embedded 3D IMEs and its applicability for biological fluid-based diagnosis.
AB - This study focused on fabricating a target-specific electrical biosensor with high sensitivity and dynamic range by embedding a hydrogel, that incorporated a much larger number of antibodies in the reaction region of the sensor, allowing all biomolecules except the target molecules to be filtered off. The biosensor is comprised of a three-dimensional interdigitated microelectrode (3D IMEs) with much higher aspect ratios than conventional IMEs and a hydrogel with a 3D mesh structure. The hydrogel increases the number of antibodies in the reaction region of the 3D IMEs by approximately 1,000-fold and enhances the impedance change induced by the specific binding of biomolecules. The hydrogel density is altered according to the target biomolecules. The impedance change by Aβ42 binding was maximized to approximately 12.135 ± 0.903% in the 3D IMEs with a ‘dense’ hydrogel. The change by prostate-specific antigen (PSA) binding was maximized in the 3D IMEs with a ‘loose’ hydrogel. Thus, the dynamic range and sensitivity for detecting Aβ42 were enhanced by a maximum of two orders and by approximately 2.58-fold compared to planar IMEs. These results demonstrate the high performance of the hydrogel-embedded 3D IMEs and its applicability for biological fluid-based diagnosis.
KW - Amyloid beta
KW - High sensitivity and selectivity
KW - Porosity tunable hydrogel
KW - Three-dimensional interdigitated microelectrodes
UR - http://www.scopus.com/inward/record.url?scp=85072670535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072670535&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.127190
DO - 10.1016/j.snb.2019.127190
M3 - Article
AN - SCOPUS:85072670535
VL - 302
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
M1 - 127190
ER -