Highly time-resolved metabolic reprogramming toward differential levels of phosphate in Chlamydomonas reinhardtii

Cheol Ho Jang, Gayeon Lee, Yong Cheol Park, Kyoung Heon Kim, Do Yup Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Understanding phosphorus metabolism in photosynthetic organisms is important as it is closely associated with enhanced crop productivity and pollution management for natural ecosystems (e.g., algal blooming). Accordingly, we exploited highly time-resolved metabolic responses to different levels of phosphate deprivation in Chlamydomonas reinhardtii, a photosynthetic model organism. We conducted non-targeted primary metabolite profiling using gas-chromatography time-of-flight mass spectrometric analysis. Primarily, we systematically identified main contributors to degree-wise responses corresponding to the levels of phosphate deprivation. Additionally, we systematically characterized the metabolite sets specific to different phosphate conditions and their interactions with culture time. Among them were various types of fatty acids that were most dynamically modulated by the phosphate availability and culture time in addition to phosphorylated compounds.

Original languageEnglish
Pages (from-to)1150-1156
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number6
DOIs
Publication statusPublished - 2017 Jun 28

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Chlamydomonas reinhardtii
Phosphates
Gas Chromatography
Phosphorus
Ecosystem
Fatty Acids

Keywords

  • Chlamydomonas reinhardtii
  • Dose responsiveness
  • GC-TOF MS
  • Phosphorus
  • Temporal resolution

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Highly time-resolved metabolic reprogramming toward differential levels of phosphate in Chlamydomonas reinhardtii. / Jang, Cheol Ho; Lee, Gayeon; Park, Yong Cheol; Kim, Kyoung Heon; Lee, Do Yup.

In: Journal of Microbiology and Biotechnology, Vol. 27, No. 6, 28.06.2017, p. 1150-1156.

Research output: Contribution to journalArticle

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