Hip2 interacts with and destabilizes Smac/DIABLO

Yoonhee Bae, Chang Won Kho, Soo Young Lee, Hyangshuk Rhim, Seong Man Kang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hip2 is a ubiquitin-conjugating enzyme that is involved in the cell cycle and suppression of cell death. To understand its role further, we tried to identify proteins that interact with Hip2. Using the immunoprecipitation technique and one-dimensional gel electrophoresis, we identified Smac/DIABLO, a proapoptotic molecule, as a protein that interacts with Hip2. The interaction of Hip2 and Smac was confirmed through in vivo and in vitro experiments. Hip2 promoted degradation of mature Smac through the ubiquitin proteasome pathway. As a result, Hip2 significantly blocked cell death induced by staurosporine and Smac. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis.

Original languageEnglish
Pages (from-to)718-723
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number4
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Cell death
Cell Death
Ubiquitin-Conjugating Enzymes
Staurosporine
Proteasome Endopeptidase Complex
Ubiquitin
Electrophoresis
Immunoprecipitation
Cell Cycle
Proteins
Gels
Cells
Apoptosis
Degradation
Molecules
Experiments
In Vitro Techniques

Keywords

  • Apoptosis
  • Hip2
  • Smac
  • Ubiquitination

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Hip2 interacts with and destabilizes Smac/DIABLO. / Bae, Yoonhee; Kho, Chang Won; Lee, Soo Young; Rhim, Hyangshuk; Kang, Seong Man.

In: Biochemical and Biophysical Research Communications, Vol. 397, No. 4, 01.07.2010, p. 718-723.

Research output: Contribution to journalArticle

Bae, Yoonhee ; Kho, Chang Won ; Lee, Soo Young ; Rhim, Hyangshuk ; Kang, Seong Man. / Hip2 interacts with and destabilizes Smac/DIABLO. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 397, No. 4. pp. 718-723.
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