Hip2 interacts with and destabilizes Smac/DIABLO

Yoonhee Bae, Chang Won Kho, Soo Young Lee, Hyangshuk Rhim, Seongman Kang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Hip2 is a ubiquitin-conjugating enzyme that is involved in the cell cycle and suppression of cell death. To understand its role further, we tried to identify proteins that interact with Hip2. Using the immunoprecipitation technique and one-dimensional gel electrophoresis, we identified Smac/DIABLO, a proapoptotic molecule, as a protein that interacts with Hip2. The interaction of Hip2 and Smac was confirmed through in vivo and in vitro experiments. Hip2 promoted degradation of mature Smac through the ubiquitin proteasome pathway. As a result, Hip2 significantly blocked cell death induced by staurosporine and Smac. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis.

Original languageEnglish
Pages (from-to)718-723
Number of pages6
JournalBiochemical and biophysical research communications
Volume397
Issue number4
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Apoptosis
  • Hip2
  • Smac
  • Ubiquitination

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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