Histone deacetylase 1 contributes to cell cycle and apoptosis

Ai Guo Wang, Sun Uk Kim, Sang Ho Lee, Sang Keun Kim, Sang Beom Seo, Dae Yeul Yu, Dong Seok Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Histone deacetylases (HDACs) are generally thought to play important roles in human disease. However, little information is available concerning the specific functions of individual HDACs. We previously reported on transgenic mice that expressed human HDAC1 and experienced steatosis and nuclear pleomorphism in their hepatic tissues. To find out if the over-expression of HDAC1 contributes to the expression of genes related to the cell cycle, apoptosis, and lipid metabolism that eventually contribute to the pathological changes in the livers of the transgenic mice, the expression profiles of the related genes in liver tissues were determined by reverse transcription- polymerase chain reaction (RT-PCR) and Western blot analysis. The activated human HDAC1 significantly induced the expression levels of mRNA for p53, PPAR-gamma and Bak and reduced the p21 expression level compared with the levels in control littermates. However, the protein levels of p53 and PPAR-gamma were significantly decreased. In conclusion, our results indicate that HDAC1 can regulate gene expression at the mRNA and protein levels independently and that this may be a potential cytopathic factor for hepatic tissue in transgenic mice that over-express HDAC1.

Original languageEnglish
Pages (from-to)1966-1970
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number10
DOIs
Publication statusPublished - 2005 Oct 1

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Histone Deacetylase 1
Cell Cycle
Apoptosis
Transgenic Mice
Histone Deacetylases
PPAR gamma
Liver
Gene Expression
Messenger RNA
Thromboplastin
Transcriptome
Lipid Metabolism
Reverse Transcription
Proteins
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Wang, A. G., Kim, S. U., Lee, S. H., Kim, S. K., Seo, S. B., Yu, D. Y., & Lee, D. S. (2005). Histone deacetylase 1 contributes to cell cycle and apoptosis. Biological and Pharmaceutical Bulletin, 28(10), 1966-1970. https://doi.org/10.1248/bpb.28.1966

Histone deacetylase 1 contributes to cell cycle and apoptosis. / Wang, Ai Guo; Kim, Sun Uk; Lee, Sang Ho; Kim, Sang Keun; Seo, Sang Beom; Yu, Dae Yeul; Lee, Dong Seok.

In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 10, 01.10.2005, p. 1966-1970.

Research output: Contribution to journalArticle

Wang, AG, Kim, SU, Lee, SH, Kim, SK, Seo, SB, Yu, DY & Lee, DS 2005, 'Histone deacetylase 1 contributes to cell cycle and apoptosis', Biological and Pharmaceutical Bulletin, vol. 28, no. 10, pp. 1966-1970. https://doi.org/10.1248/bpb.28.1966
Wang, Ai Guo ; Kim, Sun Uk ; Lee, Sang Ho ; Kim, Sang Keun ; Seo, Sang Beom ; Yu, Dae Yeul ; Lee, Dong Seok. / Histone deacetylase 1 contributes to cell cycle and apoptosis. In: Biological and Pharmaceutical Bulletin. 2005 ; Vol. 28, No. 10. pp. 1966-1970.
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