Histone deacetylase 1 contributes to cell cycle and apoptosis

Ai Guo Wang, Sun Uk Kim, Sang Ho Lee, Sang Keun Kim, Sang Beom Seo, Dae Yeul Yu, Dong Seok Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Histone deacetylases (HDACs) are generally thought to play important roles in human disease. However, little information is available concerning the specific functions of individual HDACs. We previously reported on transgenic mice that expressed human HDAC1 and experienced steatosis and nuclear pleomorphism in their hepatic tissues. To find out if the over-expression of HDAC1 contributes to the expression of genes related to the cell cycle, apoptosis, and lipid metabolism that eventually contribute to the pathological changes in the livers of the transgenic mice, the expression profiles of the related genes in liver tissues were determined by reverse transcription- polymerase chain reaction (RT-PCR) and Western blot analysis. The activated human HDAC1 significantly induced the expression levels of mRNA for p53, PPAR-gamma and Bak and reduced the p21 expression level compared with the levels in control littermates. However, the protein levels of p53 and PPAR-gamma were significantly decreased. In conclusion, our results indicate that HDAC1 can regulate gene expression at the mRNA and protein levels independently and that this may be a potential cytopathic factor for hepatic tissue in transgenic mice that over-express HDAC1.

Original languageEnglish
Pages (from-to)1966-1970
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number10
DOIs
Publication statusPublished - 2005 Oct

Keywords

  • Apoptosis
  • Cell cycle
  • Histone deacetylase 1
  • Steatosis

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Histone deacetylase 1 contributes to cell cycle and apoptosis'. Together they form a unique fingerprint.

  • Cite this

    Wang, A. G., Kim, S. U., Lee, S. H., Kim, S. K., Seo, S. B., Yu, D. Y., & Lee, D. S. (2005). Histone deacetylase 1 contributes to cell cycle and apoptosis. Biological and Pharmaceutical Bulletin, 28(10), 1966-1970. https://doi.org/10.1248/bpb.28.1966