Histone methylation-associated transgenerational inheritance of reproductive defects in Caenorhabditis elegans exposed to crude oil under various exposure scenarios

Jisu Yang, Nivedita Chatterjee, Youngho Kim, Ji Yeon Roh, Jung-Hwan Kwon, Myung Sook Park, Jinhee Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

As part of a study to explore the long-term effects of the Hebei Spirit oil spill accident, transgenerational toxicity and associated epigenetic changes were investigated in the nematode Caenorhabditis elegans. Under experimental conditions, worms were exposed to Iranian heavy crude oil (IHC) under three different scenarios: partial early-life exposure (PE), partial late-life exposure (PL), and whole-life exposure (WE). Growth, reproduction, and histone methylation were monitored in the exposed parental worms (P0) and in three consecutive unexposed offspring generations (F1-3). Reproductive potential in the exposed P0 generation in the WE treatment group was reduced; additionally, it was inhibited in the unexposed offspring generations of the P0 worms. This suggests that there was transgenerational inheritance of defective reproduction. Comparison of developmental periods of exposure showed that IHC-treated worms in the PL group had a greater reduction in reproductive capacity than those in the PE group. Decreased methylation of histone H3 (H3K9) was found in the IHC-exposed parental generation. A heritable reduction in reproductive capacity occurred in wildtype N2 but was not found in a H3K9 histone methyltransferase (HMT) mutant, met-2(n4256), suggesting a potential role for HMT in transgenerational toxicity. Our results suggest that the reproductive toxicity after IHC exposure could be heritable and that histone methylation is associated with the transmission of the inherited phenotype.

Original languageEnglish
Pages (from-to)358-365
Number of pages8
JournalChemosphere
Volume200
DOIs
Publication statusPublished - 2018 Jun 1

Fingerprint

Methylation
Petroleum
methylation
Histones
defect
crude oil
Toxicity
Defects
heavy oil
Oil spills
toxicity
Accidents
reproductive potential
exposure
oil spill
accident
nematode
phenotype
histone methyltransferase

Keywords

  • Caenorhabditis elegans
  • Differential exposure
  • Histone methylation
  • Iranian heavy crude oil
  • Transgenerational effects

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Histone methylation-associated transgenerational inheritance of reproductive defects in Caenorhabditis elegans exposed to crude oil under various exposure scenarios. / Yang, Jisu; Chatterjee, Nivedita; Kim, Youngho; Roh, Ji Yeon; Kwon, Jung-Hwan; Park, Myung Sook; Choi, Jinhee.

In: Chemosphere, Vol. 200, 01.06.2018, p. 358-365.

Research output: Contribution to journalArticle

Yang, Jisu ; Chatterjee, Nivedita ; Kim, Youngho ; Roh, Ji Yeon ; Kwon, Jung-Hwan ; Park, Myung Sook ; Choi, Jinhee. / Histone methylation-associated transgenerational inheritance of reproductive defects in Caenorhabditis elegans exposed to crude oil under various exposure scenarios. In: Chemosphere. 2018 ; Vol. 200. pp. 358-365.
@article{f230db068abe423d9f92c93def87ed2d,
title = "Histone methylation-associated transgenerational inheritance of reproductive defects in Caenorhabditis elegans exposed to crude oil under various exposure scenarios",
abstract = "As part of a study to explore the long-term effects of the Hebei Spirit oil spill accident, transgenerational toxicity and associated epigenetic changes were investigated in the nematode Caenorhabditis elegans. Under experimental conditions, worms were exposed to Iranian heavy crude oil (IHC) under three different scenarios: partial early-life exposure (PE), partial late-life exposure (PL), and whole-life exposure (WE). Growth, reproduction, and histone methylation were monitored in the exposed parental worms (P0) and in three consecutive unexposed offspring generations (F1-3). Reproductive potential in the exposed P0 generation in the WE treatment group was reduced; additionally, it was inhibited in the unexposed offspring generations of the P0 worms. This suggests that there was transgenerational inheritance of defective reproduction. Comparison of developmental periods of exposure showed that IHC-treated worms in the PL group had a greater reduction in reproductive capacity than those in the PE group. Decreased methylation of histone H3 (H3K9) was found in the IHC-exposed parental generation. A heritable reduction in reproductive capacity occurred in wildtype N2 but was not found in a H3K9 histone methyltransferase (HMT) mutant, met-2(n4256), suggesting a potential role for HMT in transgenerational toxicity. Our results suggest that the reproductive toxicity after IHC exposure could be heritable and that histone methylation is associated with the transmission of the inherited phenotype.",
keywords = "Caenorhabditis elegans, Differential exposure, Histone methylation, Iranian heavy crude oil, Transgenerational effects",
author = "Jisu Yang and Nivedita Chatterjee and Youngho Kim and Roh, {Ji Yeon} and Jung-Hwan Kwon and Park, {Myung Sook} and Jinhee Choi",
year = "2018",
month = "6",
day = "1",
doi = "10.1016/j.chemosphere.2018.02.080",
language = "English",
volume = "200",
pages = "358--365",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Histone methylation-associated transgenerational inheritance of reproductive defects in Caenorhabditis elegans exposed to crude oil under various exposure scenarios

AU - Yang, Jisu

AU - Chatterjee, Nivedita

AU - Kim, Youngho

AU - Roh, Ji Yeon

AU - Kwon, Jung-Hwan

AU - Park, Myung Sook

AU - Choi, Jinhee

PY - 2018/6/1

Y1 - 2018/6/1

N2 - As part of a study to explore the long-term effects of the Hebei Spirit oil spill accident, transgenerational toxicity and associated epigenetic changes were investigated in the nematode Caenorhabditis elegans. Under experimental conditions, worms were exposed to Iranian heavy crude oil (IHC) under three different scenarios: partial early-life exposure (PE), partial late-life exposure (PL), and whole-life exposure (WE). Growth, reproduction, and histone methylation were monitored in the exposed parental worms (P0) and in three consecutive unexposed offspring generations (F1-3). Reproductive potential in the exposed P0 generation in the WE treatment group was reduced; additionally, it was inhibited in the unexposed offspring generations of the P0 worms. This suggests that there was transgenerational inheritance of defective reproduction. Comparison of developmental periods of exposure showed that IHC-treated worms in the PL group had a greater reduction in reproductive capacity than those in the PE group. Decreased methylation of histone H3 (H3K9) was found in the IHC-exposed parental generation. A heritable reduction in reproductive capacity occurred in wildtype N2 but was not found in a H3K9 histone methyltransferase (HMT) mutant, met-2(n4256), suggesting a potential role for HMT in transgenerational toxicity. Our results suggest that the reproductive toxicity after IHC exposure could be heritable and that histone methylation is associated with the transmission of the inherited phenotype.

AB - As part of a study to explore the long-term effects of the Hebei Spirit oil spill accident, transgenerational toxicity and associated epigenetic changes were investigated in the nematode Caenorhabditis elegans. Under experimental conditions, worms were exposed to Iranian heavy crude oil (IHC) under three different scenarios: partial early-life exposure (PE), partial late-life exposure (PL), and whole-life exposure (WE). Growth, reproduction, and histone methylation were monitored in the exposed parental worms (P0) and in three consecutive unexposed offspring generations (F1-3). Reproductive potential in the exposed P0 generation in the WE treatment group was reduced; additionally, it was inhibited in the unexposed offspring generations of the P0 worms. This suggests that there was transgenerational inheritance of defective reproduction. Comparison of developmental periods of exposure showed that IHC-treated worms in the PL group had a greater reduction in reproductive capacity than those in the PE group. Decreased methylation of histone H3 (H3K9) was found in the IHC-exposed parental generation. A heritable reduction in reproductive capacity occurred in wildtype N2 but was not found in a H3K9 histone methyltransferase (HMT) mutant, met-2(n4256), suggesting a potential role for HMT in transgenerational toxicity. Our results suggest that the reproductive toxicity after IHC exposure could be heritable and that histone methylation is associated with the transmission of the inherited phenotype.

KW - Caenorhabditis elegans

KW - Differential exposure

KW - Histone methylation

KW - Iranian heavy crude oil

KW - Transgenerational effects

UR - http://www.scopus.com/inward/record.url?scp=85042502677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042502677&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2018.02.080

DO - 10.1016/j.chemosphere.2018.02.080

M3 - Article

C2 - 29494917

AN - SCOPUS:85042502677

VL - 200

SP - 358

EP - 365

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -