Homer1 regulates the susceptibility to TRAIL

Jin Na Shin, Sujan Piya, Cheol-Won Yun, Dai Wu Seol, Young Woo Seo, Ae Ran Moon, Tae Hyoung Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

TRAIL is an apoptotic cell death-inducing ligand that belongs to a TNF superfamily. To identify the regulators that govern the susceptibility to TRAIL, TRAIL-resistant HeLa (TR) cells were established by repeatedly treating HeLa cells with TRAIL. Here we showed that scaffolding protein Homer1 plays a decisive role in regulating the apoptotic susceptibility to TRAIL. TR cells showing the normal susceptibility to FasL and chemotherapeutic agent etoposide expressed the lower protein levels of Homer1 than parental HeLa cells. They showed the delayed activation of caspases-8, Bid cleavage and Bax translocation to mitochondria in response to TRAIL. Reconstitution of Homer1 expression in TR cells significantly restored the susceptibility to TRAIL. In addition, knock-down of Homer1 using interfering shRNA in parental HeLa cells lost the susceptibility to TRAIL. Together, our data indicate that Homer1 plays a critical role in determining the apoptotic susceptibility to TRAIL.

Original languageEnglish
Pages (from-to)2249-2255
Number of pages7
JournalExperimental Cell Research
Volume315
Issue number13
DOIs
Publication statusPublished - 2009 Aug 1

Fingerprint

HeLa Cells
Caspase 8
Etoposide
Small Interfering RNA
Mitochondria
Proteins
Cell Death
Ligands

Keywords

  • Apoptosis
  • Homer1
  • TRAIL

ASJC Scopus subject areas

  • Cell Biology

Cite this

Shin, J. N., Piya, S., Yun, C-W., Seol, D. W., Seo, Y. W., Moon, A. R., & Kim, T. H. (2009). Homer1 regulates the susceptibility to TRAIL. Experimental Cell Research, 315(13), 2249-2255. https://doi.org/10.1016/j.yexcr.2009.04.004

Homer1 regulates the susceptibility to TRAIL. / Shin, Jin Na; Piya, Sujan; Yun, Cheol-Won; Seol, Dai Wu; Seo, Young Woo; Moon, Ae Ran; Kim, Tae Hyoung.

In: Experimental Cell Research, Vol. 315, No. 13, 01.08.2009, p. 2249-2255.

Research output: Contribution to journalArticle

Shin, JN, Piya, S, Yun, C-W, Seol, DW, Seo, YW, Moon, AR & Kim, TH 2009, 'Homer1 regulates the susceptibility to TRAIL', Experimental Cell Research, vol. 315, no. 13, pp. 2249-2255. https://doi.org/10.1016/j.yexcr.2009.04.004
Shin JN, Piya S, Yun C-W, Seol DW, Seo YW, Moon AR et al. Homer1 regulates the susceptibility to TRAIL. Experimental Cell Research. 2009 Aug 1;315(13):2249-2255. https://doi.org/10.1016/j.yexcr.2009.04.004
Shin, Jin Na ; Piya, Sujan ; Yun, Cheol-Won ; Seol, Dai Wu ; Seo, Young Woo ; Moon, Ae Ran ; Kim, Tae Hyoung. / Homer1 regulates the susceptibility to TRAIL. In: Experimental Cell Research. 2009 ; Vol. 315, No. 13. pp. 2249-2255.
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