Homer1 regulates the susceptibility to TRAIL

Jin Na Shin, Sujan Piya, Cheol Won Yun, Dai Wu Seol, Young Woo Seo, Ae Ran Moon, Tae Hyoung Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

TRAIL is an apoptotic cell death-inducing ligand that belongs to a TNF superfamily. To identify the regulators that govern the susceptibility to TRAIL, TRAIL-resistant HeLa (TR) cells were established by repeatedly treating HeLa cells with TRAIL. Here we showed that scaffolding protein Homer1 plays a decisive role in regulating the apoptotic susceptibility to TRAIL. TR cells showing the normal susceptibility to FasL and chemotherapeutic agent etoposide expressed the lower protein levels of Homer1 than parental HeLa cells. They showed the delayed activation of caspases-8, Bid cleavage and Bax translocation to mitochondria in response to TRAIL. Reconstitution of Homer1 expression in TR cells significantly restored the susceptibility to TRAIL. In addition, knock-down of Homer1 using interfering shRNA in parental HeLa cells lost the susceptibility to TRAIL. Together, our data indicate that Homer1 plays a critical role in determining the apoptotic susceptibility to TRAIL.

Original languageEnglish
Pages (from-to)2249-2255
Number of pages7
JournalExperimental Cell Research
Volume315
Issue number13
DOIs
Publication statusPublished - 2009 Aug 1

Keywords

  • Apoptosis
  • Homer1
  • TRAIL

ASJC Scopus subject areas

  • Cell Biology

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    Shin, J. N., Piya, S., Yun, C. W., Seol, D. W., Seo, Y. W., Moon, A. R., & Kim, T. H. (2009). Homer1 regulates the susceptibility to TRAIL. Experimental Cell Research, 315(13), 2249-2255. https://doi.org/10.1016/j.yexcr.2009.04.004