Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer

Hwanseok Jang, Jacob Notbohm, Bomi Gweon, Youngbin Cho, Chan Young Park, Sun-Ho Kee, Jeffrey J. Fredberg, Jennifer H. Shin, Yongdoo Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) induces cell migration and scattering by mechanisms that are thought to tip a local balance of competing physical forces; cell-To-cell and cell-To-substrate forces. In this local process, HGF is known to attenuate local cadherin-dependent adhesion forces for cell-cell junction development and enhance local integrin-dependent contractile forces for pulling neighboring cells apart. Here we use an expanding island of confluent Madin-Darby canine kidney (MDCK) cells as a model system to quantify the collective cell migration. In the absence of HGF, cell trajectories are highly tortuous whereas in the presence of HGF, they become far less so, resembling free expansion of a gas. At the level of cell-To-cell junctions, HGF attenuates the linkage of stress fibers to cell-To-cell junctions with concomitant decrease in intercellular stress. At the level of cell-To-substrate junctions, HGF augments the linkage of stress fibers to cell-To-substrate junctions with no apparent effect on traction. Together, HGF induces both structural changes in the actin-bound junctional protein complex and physical forces spanning multicellular clusters, which further promotes the expansion of confluent cellular layer.

Original languageEnglish
Article number45844
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 2017 Apr 4

Fingerprint

Hepatocyte Growth Factor
Intercellular Junctions
Stress Fibers
Cell Movement
Madin Darby Canine Kidney Cells
Traction
Cadherins
Cell Adhesion
Integrins
Actins
Gases

ASJC Scopus subject areas

  • General

Cite this

Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer. / Jang, Hwanseok; Notbohm, Jacob; Gweon, Bomi; Cho, Youngbin; Park, Chan Young; Kee, Sun-Ho; Fredberg, Jeffrey J.; Shin, Jennifer H.; Park, Yongdoo.

In: Scientific Reports, Vol. 8, 45844, 04.04.2017.

Research output: Contribution to journalArticle

Jang, Hwanseok ; Notbohm, Jacob ; Gweon, Bomi ; Cho, Youngbin ; Park, Chan Young ; Kee, Sun-Ho ; Fredberg, Jeffrey J. ; Shin, Jennifer H. ; Park, Yongdoo. / Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer. In: Scientific Reports. 2017 ; Vol. 8.
@article{2fd2f8ae797349efaef141216513e9fb,
title = "Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer",
abstract = "Hepatocyte growth factor (HGF) induces cell migration and scattering by mechanisms that are thought to tip a local balance of competing physical forces; cell-To-cell and cell-To-substrate forces. In this local process, HGF is known to attenuate local cadherin-dependent adhesion forces for cell-cell junction development and enhance local integrin-dependent contractile forces for pulling neighboring cells apart. Here we use an expanding island of confluent Madin-Darby canine kidney (MDCK) cells as a model system to quantify the collective cell migration. In the absence of HGF, cell trajectories are highly tortuous whereas in the presence of HGF, they become far less so, resembling free expansion of a gas. At the level of cell-To-cell junctions, HGF attenuates the linkage of stress fibers to cell-To-cell junctions with concomitant decrease in intercellular stress. At the level of cell-To-substrate junctions, HGF augments the linkage of stress fibers to cell-To-substrate junctions with no apparent effect on traction. Together, HGF induces both structural changes in the actin-bound junctional protein complex and physical forces spanning multicellular clusters, which further promotes the expansion of confluent cellular layer.",
author = "Hwanseok Jang and Jacob Notbohm and Bomi Gweon and Youngbin Cho and Park, {Chan Young} and Sun-Ho Kee and Fredberg, {Jeffrey J.} and Shin, {Jennifer H.} and Yongdoo Park",
year = "2017",
month = "4",
day = "4",
doi = "10.1038/srep45844",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer

AU - Jang, Hwanseok

AU - Notbohm, Jacob

AU - Gweon, Bomi

AU - Cho, Youngbin

AU - Park, Chan Young

AU - Kee, Sun-Ho

AU - Fredberg, Jeffrey J.

AU - Shin, Jennifer H.

AU - Park, Yongdoo

PY - 2017/4/4

Y1 - 2017/4/4

N2 - Hepatocyte growth factor (HGF) induces cell migration and scattering by mechanisms that are thought to tip a local balance of competing physical forces; cell-To-cell and cell-To-substrate forces. In this local process, HGF is known to attenuate local cadherin-dependent adhesion forces for cell-cell junction development and enhance local integrin-dependent contractile forces for pulling neighboring cells apart. Here we use an expanding island of confluent Madin-Darby canine kidney (MDCK) cells as a model system to quantify the collective cell migration. In the absence of HGF, cell trajectories are highly tortuous whereas in the presence of HGF, they become far less so, resembling free expansion of a gas. At the level of cell-To-cell junctions, HGF attenuates the linkage of stress fibers to cell-To-cell junctions with concomitant decrease in intercellular stress. At the level of cell-To-substrate junctions, HGF augments the linkage of stress fibers to cell-To-substrate junctions with no apparent effect on traction. Together, HGF induces both structural changes in the actin-bound junctional protein complex and physical forces spanning multicellular clusters, which further promotes the expansion of confluent cellular layer.

AB - Hepatocyte growth factor (HGF) induces cell migration and scattering by mechanisms that are thought to tip a local balance of competing physical forces; cell-To-cell and cell-To-substrate forces. In this local process, HGF is known to attenuate local cadherin-dependent adhesion forces for cell-cell junction development and enhance local integrin-dependent contractile forces for pulling neighboring cells apart. Here we use an expanding island of confluent Madin-Darby canine kidney (MDCK) cells as a model system to quantify the collective cell migration. In the absence of HGF, cell trajectories are highly tortuous whereas in the presence of HGF, they become far less so, resembling free expansion of a gas. At the level of cell-To-cell junctions, HGF attenuates the linkage of stress fibers to cell-To-cell junctions with concomitant decrease in intercellular stress. At the level of cell-To-substrate junctions, HGF augments the linkage of stress fibers to cell-To-substrate junctions with no apparent effect on traction. Together, HGF induces both structural changes in the actin-bound junctional protein complex and physical forces spanning multicellular clusters, which further promotes the expansion of confluent cellular layer.

UR - http://www.scopus.com/inward/record.url?scp=85017135760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017135760&partnerID=8YFLogxK

U2 - 10.1038/srep45844

DO - 10.1038/srep45844

M3 - Article

C2 - 28374776

AN - SCOPUS:85017135760

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 45844

ER -