Abstract
We report on another alternative sensing platform for the detection of protein biomarker (PSA-ACT complex) based on homogenous growth of Au nanocrystals in solution phase. The immuno-recognition event is translated into the gold nanoparticle growth signal which can be intuitively recognized by an unaided eye, or quantitatively measured by an UV-vis spectrophotometric analysis. Surface plasmonic signature and kinetics of the Au nanogrowth in the homogenous phase containing of HAuCl4, AA, and CTAB have also been studied to provide suitable parameters for the immunoassay. As a result, detection limit of PSA-ACT complex was determined to be 10 fM. The result indicated that this is a very sensitive, robust, simple, and economic strategy to detect protein biomarkers, and it has great potential to detect other biological interactions.
| Original language | English |
|---|---|
| Pages (from-to) | 1292-1297 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
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Keywords
- Gold nanoparticles
- Homogenous nanogrowth
- Magnetic separation
- Protein biomarker
- Solution phase
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry
Cite this
Homogenous growth of gold nanocrystals for quantification of PSA protein biomarker. / Cao, Cuong; Li, Xinxing; Lee, Jeewon; Sim, Sang Jun.
In: Biosensors and Bioelectronics, Vol. 24, No. 5, 01.01.2009, p. 1292-1297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homogenous growth of gold nanocrystals for quantification of PSA protein biomarker
AU - Cao, Cuong
AU - Li, Xinxing
AU - Lee, Jeewon
AU - Sim, Sang Jun
PY - 2009/1/1
Y1 - 2009/1/1
N2 - We report on another alternative sensing platform for the detection of protein biomarker (PSA-ACT complex) based on homogenous growth of Au nanocrystals in solution phase. The immuno-recognition event is translated into the gold nanoparticle growth signal which can be intuitively recognized by an unaided eye, or quantitatively measured by an UV-vis spectrophotometric analysis. Surface plasmonic signature and kinetics of the Au nanogrowth in the homogenous phase containing of HAuCl4, AA, and CTAB have also been studied to provide suitable parameters for the immunoassay. As a result, detection limit of PSA-ACT complex was determined to be 10 fM. The result indicated that this is a very sensitive, robust, simple, and economic strategy to detect protein biomarkers, and it has great potential to detect other biological interactions.
AB - We report on another alternative sensing platform for the detection of protein biomarker (PSA-ACT complex) based on homogenous growth of Au nanocrystals in solution phase. The immuno-recognition event is translated into the gold nanoparticle growth signal which can be intuitively recognized by an unaided eye, or quantitatively measured by an UV-vis spectrophotometric analysis. Surface plasmonic signature and kinetics of the Au nanogrowth in the homogenous phase containing of HAuCl4, AA, and CTAB have also been studied to provide suitable parameters for the immunoassay. As a result, detection limit of PSA-ACT complex was determined to be 10 fM. The result indicated that this is a very sensitive, robust, simple, and economic strategy to detect protein biomarkers, and it has great potential to detect other biological interactions.
KW - Gold nanoparticles
KW - Homogenous nanogrowth
KW - Magnetic separation
KW - Protein biomarker
KW - Solution phase
UR - http://www.scopus.com/inward/record.url?scp=58149172059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149172059&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2008.07.046
DO - 10.1016/j.bios.2008.07.046
M3 - Article
C2 - 18783934
AN - SCOPUS:58149172059
VL - 24
SP - 1292
EP - 1297
JO - Biosensors
JF - Biosensors
SN - 0956-5663
IS - 5
ER -