Homogenous growth of gold nanocrystals for quantification of PSA protein biomarker

Cuong Cao; Xinxing Li; Jeewon Lee; Sang Jun Sim

doi:10.1016/j.bios.2008.07.046

Homogenous growth of gold nanocrystals for quantification of PSA protein biomarker

Cuong Cao, Xinxing Li, Jeewon Lee, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

We report on another alternative sensing platform for the detection of protein biomarker (PSA-ACT complex) based on homogenous growth of Au nanocrystals in solution phase. The immuno-recognition event is translated into the gold nanoparticle growth signal which can be intuitively recognized by an unaided eye, or quantitatively measured by an UV-vis spectrophotometric analysis. Surface plasmonic signature and kinetics of the Au nanogrowth in the homogenous phase containing of HAuCl₄, AA, and CTAB have also been studied to provide suitable parameters for the immunoassay. As a result, detection limit of PSA-ACT complex was determined to be 10 fM. The result indicated that this is a very sensitive, robust, simple, and economic strategy to detect protein biomarkers, and it has great potential to detect other biological interactions.

Original language	English
Pages (from-to)	1292-1297
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2008.07.046
Publication status	Published - 2009 Jan 1

Keywords

Gold nanoparticles
Homogenous nanogrowth
Magnetic separation
Protein biomarker
Solution phase

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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